FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2127923
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC83010.3900987018797203No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC68910.32383690575269874No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAACACTCG64010.3008097567440175No Hit
GTAATACACAGCCGAGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA56790.2668799575924505No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG56590.2659400739594431No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT50880.2391063962370819No Hit
CCCCTGTGCTGGTCATCTATGAGGACAGCAAACGACCCTCCGGGATCCCT47980.22547808355847465No Hit
GACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAACACTCGTCTATGATTA44820.21062792215695772No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG44730.2102049745221044No Hit
GCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATTTTTTT44380.20856017816434147No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC41140.19333406330962163No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG41040.19286412149311793No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT40950.1924411738582646No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC40730.19140730186195648No Hit
TCTATGAGGACAGCAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGC40650.19103134840875352No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG40330.18952753459594168No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTCGGTCCCTC40150.18868163932623502No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA39580.18600297097216392No Hit
CTACTACTGTTACTCAACAGACAGCAGTGATAATCATAGACGAGTGTTCG38820.18243141316673583No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT37910.17815494263655218No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATG37680.17707407645859366No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG36370.1709178386623952No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG35540.16701732158541452No Hit
GACTACTACTGTTACTCAACAGACAGCAGTGATAATCATAGACGAGTGTTCGGCGGAGGGACCGAGCTGACCGTC35240.1656074961359034No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGC34780.1634457637799864No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA34530.16227090923872717No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG34490.1620829325121257No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT33540.15761848525534053No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC32790.1540939216315628No Hit
GTGCTGGTCATCTATGAGGACAGCAAACGACCCTCCGGGATCCCTGAGAG32650.15343600308845762No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA32120.15094531146098802No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC31760.1492535209215747No Hit
GTGTATTACTGTGCGAGAGACATATATTACGATGTTTTGACTGGTTATGG31310.14713878274730804No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG31280.14699780020235695No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA30610.14384919003178218No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC30240.14211040531071847No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGG29940.14070057986120738No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29430.13830387659703852No Hit
GGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAGCAAACGACCCTCCGG28500.1339334177035541No Hit
CAGTAGTAGTCAGCTTCATCCTCCACCTGGGCCCCACTGATAGTCAAGGT27210.12787116827065642No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC27180.1277301857257053No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT27060.12716625554590086No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC26600.12500452318998384No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA26470.12439359882852905No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26140.12284279083406684No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA26130.12279579665241648No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26080.12256082574416462No Hit
GTAGTAGTCAGCTTCATCCTCCACCTGGGCCCCACTGATAGTCAAGGTGG26040.12237284901756312No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAACA25930.12185591301940907No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAA25620.1203990933882476No Hit
ACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAACACTCGTCTATGATTAT25370.11922423884698835No Hit
GCTCAGGGACAATGGCCACCTTGACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTACTGTTACTCAA24650.11584065776816173No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC24090.113208983595741No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC23610.11095326287652327No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT23600.11090626869487288No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC23400.1099663850618655No Hit
CTGATAGTCAAGGTGGCCATTGTCCCTGAGCTGGAGCCAGAGAATCTCTCAGGGATCCCGGAGGGTCGTTTG23230.1091674839738092No Hit
GGATGAAGCTGACTACTACTGTTACTCAACAGACAGCAGTGATAATCATA23060.1083685828857529No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA22820.10724072252614404No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT22640.10639482725643737No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTCGGTC22240.10451505999042258No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT22110.10390413562896778No Hit
GACCAGCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATT21560.10131945563819744No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT21420.10066153709509225No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG21330.10023858946023893No Hit
GCTCTGGAGATGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAG21320.10019159527858855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG7600.073.77627670
ATCGCGT750.059.25494837
CGCGTAT800.055.76651839
GCGTATG1300.050.25411640
GGTATCA15150.046.803361
CGTATGC8500.045.81300741
GTATGCC8850.044.87579342
ATGCCGT8400.044.17881844
TCTCGTA6000.043.9540938
ACTCGAC12550.043.9040427
GTGGTAT3850.043.8510171
TCGACGT12350.043.8444629
GGCGTAT551.5330261E-743.67727339
CTTATAC12250.043.5503161
CGACGTC12400.043.43055730
TCGTATG8500.043.28388640
GACTCGA12650.043.27855326
GCCGTCT7900.043.23045346
TTATACA12350.043.1976852
CCGTCTT8050.042.95474247
Produced by FastQC (version 0.11.5)