FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421601
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGTGGGTCCTTCAGTGATTACTACTGGAGCTGGATCCGCCAGCC52810.3714825749278455No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCATGGTCCCTCCGCCGAACACCCAAATATTACTACCTGCATATGAGCAG51180.36001662913855575No Hit
GACCTAGGACGGTCAGCATGGTCCCTCCGCCGAACACCCAAATATTACTACCTGCATATGAGCAGCAGTAATAAT46880.3297690420870554No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC44750.3147859350127075No Hit
GTAATACACAGCCGTGTCCGCGGCGGTCACAGACCTCAGCTTCAGGGAGA44450.3126756382416726No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG41840.29431605633366886No Hit
GATTATTACTGCTGCTCATATGCAGGTAGTAATATTTGGGTGTTCGGCGGAGGGACCATGCTGACCGTCCTAGGT40590.2855231531210234No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT39310.27651922023127445No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCACCATCTCCTG37760.26561602024759406No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT36190.25457213381251137No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGACTGTCAGGGAGG35140.24718609511388917No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCATGGTCCCTC34380.24184000996060076No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG33930.23867456480404842No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGTACCCAGGCAAAGC33770.23754907319282975No Hit
GTTATAACCTTGTCTCCTGGTACCAACAGTACCCAGGCAAAGCCCCCAAA32770.2305147506227134No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGTA32600.2293189157857936No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA31870.22418386030960868No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCAC31150.21911914805912489No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT30660.21567232999976788No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT30000.21102967710349108No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG29820.20976349904087013No Hit
GACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCACCATCT29640.20849732097824916No Hit
GTACCAACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTTTGAGGGCA29370.20659805388431773No Hit
CCCAAATATTACTACCTGCATATGAGCAGCAGTAATAATCAGCCTCGTCC29300.20610565130440964No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28990.20392501130767354No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG28260.19878995583148856No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC27830.19576519712633855No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27570.19393627325810828No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG27330.19224803584128036No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA26340.18528405649686516No Hit
GTGTATTACTGTGCGAGAGGCCGTGGTGGATACACCTATGAGACGTTCGA25760.18120414940619767No Hit
CCCTGACAGTCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGC25230.177475958444036No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24620.173185021676265No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG24190.17016026297111495No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24020.16896442813419518No Hit
GTCAGGGAGGCCGTGTTGGCAGACTTGGAGCCAGAGAAGCGATTAGAAAC23940.16840168232858588No Hit
GCATGGTCCCTCCGCCGAACACCCAAATATTACTACCTGCATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAG23360.16432177523791835No Hit
CTGTCAGGGAGGCCGTGTTGGCAGACTTGGAGCCAGAGAAGCGATTAGAAACCCCTGAGGGCCGCTTACTGCCCT22880.16094530040426253No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22690.1596087791159404No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC22180.15602127460518106No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCAC21610.15201171074021474No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGA21490.15116759203180077No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC21320.14997175719488098No Hit
CCCCTGAGGGCCGCTTACTGCCCTCAAAAATCATGAGTTTGGGGGCTTTGCCTGGGTACTGTTGGTACCAGGAGA20870.1468063120383286No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20620.14504773139579952No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20530.14441464236448906No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20450.14385189655887975No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA20180.14195262946494833No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCATGGTCCCTCCGCCGAACAC20030.14089748107943087No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19590.13780237914857968No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC19230.1352700230233378No Hit
ACCTAGGACGGTCAGCATGGTCCCTCCGCCGAACACCCAAATATTACTAC19180.13491830689483197No Hit
GGTACCAACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTTTGAGGGC19150.13470727721772846No Hit
ACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTTTGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTT18840.1325266372209924No Hit
CTGTTGGTACCAGGAGACAAGGTTATAACTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGA18740.13182320496398076No Hit
GGTTATAACTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATC18580.13069771335276212No Hit
GCCTGGAGCCCAGAGACTGTCAGGGAGGCCGTGTTGGCAGACTTGGAGCC18460.12985359464434817No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC18090.1272508952934051No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCATGGTCCCTCCGCCGAACACCCAAATATTA17570.12359304755694459No Hit
CAACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTTTGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCG17260.12141240756020852No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC17160.1207089753031969No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17030.11979451336908177No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16990.1195131404662771No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC16940.11916142433777129No Hit
CAGTAATACACAGCCGTGTCCGCGGCGGTCACAGACCTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCTA16940.11916142433777129No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT16900.11888005143496665No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16700.11747318692094336No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT16680.11733250046954104No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT16580.11662906821252939No Hit
CTCCCTGACAGTCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACT16520.11620700885832241No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCG16280.1145187714414945No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16280.1145187714414945No Hit
ATATTTGGGTGTTCGGCGGAGGGACCATGCTGACCGTCCTAGGTCAGCCC16100.11325259337887354No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC16080.11311190692747121No Hit
GTCCCTCCGCCGAACACCCAAATATTACTACCTGCATATGAGCAGCAGTA15790.11107195338213748No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC15410.10839891080549324No Hit
GTCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATA15390.10825822435409091No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15360.10804719467698742No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15350.10797685145128627No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15200.10692170306576881No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGCCAACACGGCCTCCCTGACAGTCTCTGGGCTCCAGG15160.10664033016296415No Hit
GGGTACTGTTGGTACCAGGAGACAAGGTTATAACTCCCAACATCACTGCT15140.10649964371156181No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG14990.10544449532604437No Hit
GTACTGTTGGTACCAGGAGACAAGGTTATAACTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGA14980.1053741521003432No Hit
GCCCAGAGACTGTCAGGGAGGCCGTGTTGGCAGACTTGGAGCCAGAGAAG14840.10438934694052691No Hit
GCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCACCATCTCCTGCA14830.10431900371482573No Hit
GTATCAACGCAGAGTACGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG14760.10382660113491761No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14720.10354522823211294No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA14700.10340454178071061No Hit
GGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT14590.10263076629799782No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14480.10185699081528501No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC14380.10115355855827338No Hit
GTACCCAGGCAAAGCCCCCAAACTCATGATTTTTGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCT14250.10023909662415825No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGACT200.00647538553.4519463
TGGTATC3200.049.1202132
GTGGTAT3200.048.0540731
TGTCGTA451.8820374E-645.92749838
TGGATTG18450.044.3783970
TACTGAT15400.043.2550170
GGTATCA10150.042.7568131
GTATCAA28350.040.620431
GCGAATC1950.040.48032833
GACAGGT2550.038.862031
AGGGGGG2350.038.38498770
TCTCGTA1550.037.77906838
TCGTATG1950.037.17305440
CTTATAC2950.037.06771
GGCGAAT2500.037.03188332
TATCAAC31350.036.7320142
ATCTCGT1600.036.57124337
ATGCCGT1950.035.5519744
CACGAGA2800.035.3884721
AATCTCG1750.035.3783536
Produced by FastQC (version 0.11.5)