FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421601
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGTGGGTCCTTCAGTGATTACTACTGGAGCTGGATCCGCCAGCC48680.3424308227132648No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCATGGTCCCTCCGCCGAACACCCAAATATTACTACCTGCATATGAGCAG45460.31978030403749014No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC44320.31176117630755745No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG44140.3104949982449365No Hit
GTAATACACAGCCGTGTCCGCGGCGGTCACAGACCTCAGCTTCAGGGAGA41930.29494914536497935No Hit
GACCTAGGACGGTCAGCATGGTCCCTCCGCCGAACACCCAAATATTACTACCTGCATATGAGCAGCAGTAATAAT41500.2919243866598293No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCACCATCTCCTG37430.2632946937994557No Hit
GATTATTACTGCTGCTCATATGCAGGTAGTAATATTTGGGTGTTCGGCGGAGGGACCATGCTGACCGTCCTAGGT36300.2553459092952242No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT35560.2501405105933381No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT32010.22516866546942493No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGTACCCAGGCAAAGC31690.22291768224698771No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGTA31520.22172184741006792No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31280.22003360999324004No Hit
GTTATAACCTTGTCTCCTGGTACCAACAGTACCCAGGCAAAGCCCCCAAA31240.21975223709043537No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCAC30830.21686816483668767No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG30610.21532061387126203No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCATGGTCCCTC29770.20941178291236429No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG29710.20898972355815731No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT28120.19780515067167231No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGACTGTCAGGGAGG27770.19534313777213155No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA27660.19456936228941876No Hit
GACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCACCATCT26820.18866053133052102No Hit
GTACCAACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTTTGAGGGCA26570.1869019506879919No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26520.1865502345594861No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26370.18549508617396865No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCATGGTCCCTCCGCCGAACAC25940.1824703274688186No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25580.1799379713435767No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG24900.1751546319958976No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24730.1739587971589778No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC24640.17332570812766734No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23970.16861271200568936No Hit
CCCAAATATTACTACCTGCATATGAGCAGCAGTAATAATCAGCCTCGTCC23950.16847202555428703No Hit
GTCAGGGAGGCCGTGTTGGCAGACTTGGAGCCAGAGAAGCGATTAGAAAC23850.1677685932972754No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA23580.165869326203344No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC23520.165447266849137No Hit
GTGTATTACTGTGCGAGAGGCCGTGGTGGATACACCTATGAGACGTTCGA23340.16418108878651605No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG22600.15897569008462994No Hit
CCCTGACAGTCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGC22450.1579205416991125No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21910.15412200751124963No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCAC21910.15412200751124963No Hit
GCATGGTCCCTCCGCCGAACACCCAAATATTACTACCTGCATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAG21850.15369994815704266No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGA21580.15180068106311123No Hit
CTGTCAGGGAGGCCGTGTTGGCAGACTTGGAGCCAGAGAAGCGATTAGAAACCCCTGAGGGCCGCTTACTGCCCT20870.1468063120383286No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20440.14378155333317857No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20420.14364086688177627No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC19470.1369582604401657No Hit
CCCCTGAGGGCCGCTTACTGCCCTCAAAAATCATGAGTTTGGGGGCTTTGCCTGGGTACTGTTGGTACCAGGAGA19360.13618448495745292No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19280.1356217391518436No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC18880.13280801012379703No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18510.130205310772854No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18460.12985359464434817No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18400.1294315352901412No Hit
GGTACCAACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTTTGAGGGC18210.12809501400181908No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18090.1272508952934051No Hit
GGTTATAACTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATC17350.122045496591519No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17240.1212717211088062No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCG17160.1207089753031969No Hit
ACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTTTGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTT17140.12056828885179456No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC16980.11944279724057595No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT16830.1183876488550585No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCATGGTCCCTCCGCCGAACACCCAAATATTA16650.11712147079243754No Hit
GGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT16620.11691044111533405No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT16620.11691044111533405No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC16500.11606632240692008No Hit
CTGTTGGTACCAGGAGACAAGGTTATAACTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGA16130.11346362305597703No Hit
CTCCCTGACAGTCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACT15840.1114236695106433No Hit
CAACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTTTGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCG15780.1110016101564363No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC15590.10966508886811419No Hit
ATATTTGGGTGTTCGGCGGAGGGACCATGCTGACCGTCCTAGGTCAGCCC15360.10804719467698742No Hit
CAGTAATACACAGCCGTGTCCGCGGCGGTCACAGACCTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCTA15260.10734376241997579No Hit
GCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCACCATCTCCTGCA15200.10692170306576881No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT15050.10586655468025136No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14930.10502243597183739No Hit
GTCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATA14900.10481140629473389No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC14810.10417831726342343No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14720.10354522823211294No Hit
ACCTAGGACGGTCAGCATGGTCCCTCCGCCGAACACCCAAATATTACTAC14620.1028417959751013No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14620.1028417959751013No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA14590.10263076629799782No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14580.10256042307229667No Hit
GTATCAACGCAGAGTACGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG14490.10192733404098618No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC14400.10129424500967571No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACAA150.002456647868.27338422
TACTGAT13700.054.1876370
CTATACG200.007679277651.1960374
GTGGTAT3450.048.513021
TGGTATC3500.047.8132442
TGGATTG18650.045.2158370
CCGTCAT803.865301E-545.04675770
GGTATCA11450.044.4490131
GCTAGAT504.120533E-640.982782
GTATCAA27400.036.5256841
TGTCTAC13400.033.1320366
TATCAAC29950.032.8409582
GTCTACT13650.032.6028267
GCTTGTT1057.166818E-1032.5305981
TCTACTG14500.032.09062668
CTACTGA16400.032.07435669
TTCTAAG652.4883426E-531.5252172
TCAACGC32000.030.824284
ATCAACG31900.030.8236733
GTGGATT19000.030.68446569
Produced by FastQC (version 0.11.5)