FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1266933
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGGGCTTACCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAGATGAAACGAACTGTGGCTGCACCAT51630.40751957680477185No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46950.37057997542095755No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45940.3626079674300062No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33880.2674174561717155No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33070.26102406362451686No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32890.2596033097251394No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29860.23568728575228526No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG29260.23095143942102697No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28580.22558414691226766No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27990.22092723135319703No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27860.21990113131475775No Hit
GCCCTATGTTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC27800.21942754668163195No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26280.20743006930911104No Hit
GTCTACAACATAGGGCTTACCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAGATGAAACGAACTGTGGCTGCAC25950.20482535382691905No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT25820.2037992537884797No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25560.20174705371160118No Hit
CCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAGATGAAACGAACTGTGG25500.20127346907847535No Hit
GTCCAGGGGTAAGCCCTATGTTGTAGACAGTAATAAGTTGCAAAATCTTC22960.18122505294281543No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22930.18098826062625253No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22370.17656813738374486No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGACATCAGCAA21380.16875399093716875No Hit
ACTTATTACTGTCTACAACATAGGGCTTACCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAGATGAAACGAA21340.1684382678484182No Hit
GATTTAGGCTGGTATCAGCGGAAAGCAGGAAAAGCCCCCAAGCGCCTGAT21310.16820147553185527No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20320.16038732908527917No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20000.15786154437527478No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19750.15588827507058384No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19540.15423072885464345No Hit
GATCTATGTTGGTTTTAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCGGTAGATCTGGGACAGAATTCA19500.1539150057658929No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19460.15359928267714235No Hit
ATGTTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGA19130.1509945671949503No Hit
GTTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGAT18890.14910022866244702No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCTACCGCCG18790.14831092094057066No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18550.14641658240806738No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18540.14633765163587972No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC18490.14594299777494152No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCTACAACATAGGGCTTAC18310.14452224387556406No Hit
CTACAACATAGGGCTTACCCCTGGACGTTCGGCCAAGGGACCAAGGTGGA18120.14302255920399895No Hit
AGTCAGGACATCAGCAATGATTTAGGCTGGTATCAGCGGAAAGCAGGAAA18070.14262790534306075No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17990.14199645916555967No Hit
GGACCAAGGTGGAGATGAAACGAACTGTGGCTGCACCATCTGTCTTCATC16950.13378765885804536No Hit
ACATAGATCAGGCGCTTGGGGGCTTTTCCTGCTTTCCGCTGATACCAGCC16920.13355086654148246No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16900.13339300499710718No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16330.12889395098241185No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16320.1288150202102242No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16040.12660495858897036No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15910.12557885855053108No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15840.12502634314521763No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15840.12502634314521763No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15570.12289521229615141No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15570.12289521229615141No Hit
GTGCAGCCACAGTTCGTTTCATCTCCACCTTGGTCCCTTGGCCGAACGTC15540.12265841997958851No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15340.12107980453583576No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15210.12005370449739647No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15010.11847508905364372No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCAGGTGTGCCAT14920.11776471210395499No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14900.11760685055957971No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14770.11658075052114042No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14590.11515999662176295No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14460.11413389658332368No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14450.11405496581113604No Hit
GGGCTTACCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAGATGAAACGA14440.1139760350389484No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATCTCCACCTTGGTCCCTTGGCCGAACGTCCAGGGGTAAGC14370.11342351963363492No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT13920.10987163488519125No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13830.10916125793550252No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13750.1085298117580014No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13740.10845088098581376No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT13660.10781943480831267No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13560.10703012708643629No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12900.10182069612205222No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12710.10032101145048711No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTTT200.005440623555.85640367
CCCGGTT200.00572333855.14589366
GTACGGT200.00779469951.0028726
TACGGTA200.007799566750.994827
TGTCGTA306.3926826E-445.88109638
GTGGTAT3650.043.788471
TCGTATG3100.041.1812240
GTATCAA35750.038.238851
CGTATGC3450.038.0599241
ACGAGAC3650.037.27414722
ATGCCGT3550.036.16097644
ATCTCGT3400.035.37203237
ATTAGAC400.00278724434.00463
CCCACGA3800.034.00057219
TCTCCGA4150.033.5816511
TATCAAC40900.033.507082
TATACAC4300.033.2137953
TACACAT4200.033.1936575
ATCAACG42100.032.631493
CCGTCTT3750.032.50507747
Produced by FastQC (version 0.11.5)