FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1266933
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGGGCTTACCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAGATGAAACGAACTGTGGCTGCACCAT45760.3611872135306287No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43450.34295420515528446No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36400.2873080107630001No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36020.2843086414198699No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32810.2589718635476383No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31990.252499540228252No Hit
GCCCTATGTTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC28990.2288203085719608No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25970.20498321537129433No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25510.201352399850663No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG25360.20016843826784841No Hit
GTCTACAACATAGGGCTTACCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAGATGAAACGAACTGTGGCTGCAC24940.19685334583596764No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24750.19535366116440253No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24240.19132819178283303No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24070.1899863686556432No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT23870.18840775321189046No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23170.18288259915875582No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22690.17909392209374925No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22680.1790149913215616No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22300.17601562197843137No Hit
CCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAGATGAAACGAACTGTGG21720.17143763719154842No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20850.16457066001122395No Hit
GTCCAGGGGTAAGCCCTATGTTGTAGACAGTAATAAGTTGCAAAATCTTC20380.160860913718405No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19390.1530467672718289No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19300.15233639032214016No Hit
ACTTATTACTGTCTACAACATAGGGCTTACCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAGATGAAACGAA19210.15162601337245143No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGACATCAGCAA19050.15036312101744922No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19030.15020525947307395No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18690.14752161321869428No Hit
GTTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGAT18620.14696909781338083No Hit
GATTTAGGCTGGTATCAGCGGAAAGCAGGAAAAGCCCCCAAGCGCCTGAT18390.14515369005306517No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18270.1442065207868135No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC18180.14349614383712478No Hit
GATCTATGTTGGTTTTAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCGGTAGATCTGGGACAGAATTCA18070.14262790534306075No Hit
ATGTTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGA17880.14112822067149566No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17880.14112822067149566No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCTACCGCCG17580.13876029750586652No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16710.13189332032554207No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCTACAACATAGGGCTTAC16580.1308672202871028No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16310.12873608943803658No Hit
AGTCAGGACATCAGCAATGATTTAGGCTGGTATCAGCGGAAAGCAGGAAA16060.12676282013334564No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15970.1260524431836569No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15700.1239213123345907No Hit
CTACAACATAGGGCTTACCCCTGGACGTTCGGCCAAGGGACCAAGGTGGA15610.12321093538490197No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15600.12313200461271433No Hit
GGACCAAGGTGGAGATGAAACGAACTGTGGCTGCACCATCTGTCTTCATC15350.1211587353080234No Hit
ACATAGATCAGGCGCTTGGGGGCTTTTCCTGCTTTCCGCTGATACCAGCC15310.12084301221927285No Hit
GGGCTTACCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAGATGAAACGA14870.1173700582430168No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCGGTAGATCTGGGACAGAATTCACT14570.11500213507738767No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14540.11476534276082476No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14530.11468641198863713No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14340.11318672731707201No Hit
GTGCAGCCACAGTTCGTTTCATCTCCACCTTGGTCCCTTGGCCGAACGTC14290.11279207345613383No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14160.11176597341769454No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14150.1116870426455069No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13970.11026628874612943No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13810.10900339639112724No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATCTCCACCTTGGTCCCTTGGCCGAACGTCCAGGGGTAAGC13800.1089244656189396No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13730.10837195021362613No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13400.10576723473143411No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13300.10497792700955773No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13150.10379396542674316No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)