FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1504112
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA56830.3778309062091121No Hit
GTATTACTGTCAGCAGTATGGGAACTCAGCTCAACGCAGTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAA43000.2858829661620943No Hit
GTGTATTACTGTGCGAGAGAGAAAGTAGCTCGGGCCTTCCTTCTAGACTA40410.2686635037816333No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35110.23342676609188678No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30930.2056362824045018No Hit
CAGTAATACACTGCAGAATCTTGAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA29150.19380205729360578No Hit
GTGTATTACTGTCAGCAGTATGGGAACTCAGCTCAACGCAGTTTTGGCCA27620.18362994245109407No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCA26430.17571829757358495No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26020.17299243673343476No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT26000.172859467911964No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC24650.16388407246268893No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23040.15318008233429425No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22550.1499223462082611No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA22520.14972289297605498No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG22370.1487256268150244No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21730.14447062452796067No Hit
GTATAGGGACTGAAGGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGACCCCCCAGGCTTGACCAGGCCTCCC21630.143805780420607No Hit
GTATGGGAACTCAGCTCAACGCAGTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT21550.14327390513472402No Hit
CATCTATGGTGCGTCCAGCAGGGTCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA21490.14287499867031178No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21260.14134585722339826No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20150.1339660876317721No Hit
GTCTCCAGGCAGCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG20020.13310179029221228No Hit
GTAATACACTGCAGAATCTTGAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA20010.1330353058814769No Hit
GTATTACTGTGCGAGAGAGAAAGTAGCTCGGGCCTTCCTTCTAGACTACTGGGGCCAGGGAACCCTGGTCACCGT20000.13296882147074154No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT19880.1321710085419171No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19780.1315061644345634No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTCTCTGCTGGTACCAGGCTAAGTAGTTGCTGCTAACACTCTGA19600.13030944504132672No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19380.12884678800514857No Hit
GTCCCTATACCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAG19040.12658631804014595No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT18940.12592147393279224No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG18800.12499069218249706No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18540.1232620975033774No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18440.1225972533960237No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18300.12166647164572851No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17490.11628123437616349No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT17470.11614826555469274No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17300.11501803057219144No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17150.11402076441116088No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACC17090.11362185794674864No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17040.1132894358930718No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC16930.1125581073749827No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16800.11169381003542289No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16770.11149435680321677No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC16770.11149435680321677No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16710.11109545033880455No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16690.11096248151733382No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTCAAGATT16250.1080371674449775No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG16160.10743880774835918No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16070.10684044805174082No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC15560.10344974310423692No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTCCCTATACCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG15420.10251896135394173No Hit
GCCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGC15230.10125575754996968No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15060.10012552256746837No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)