FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1504112
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50960.33880455710744944No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA50120.33321986660567826No Hit
GTGTATTACTGTGCGAGAGAGAAAGTAGCTCGGGCCTTCCTTCTAGACTA39930.2654722520663355No Hit
GTATTACTGTCAGCAGTATGGGAACTCAGCTCAACGCAGTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAA39330.2614831874222132No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33120.22019636835554798No Hit
CAGTAATACACTGCAGAATCTTGAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA26440.17578478198432032No Hit
GTGTATTACTGTCAGCAGTATGGGAACTCAGCTCAACGCAGTTTTGGCCA25470.16933579414298935No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25020.1663439956598977No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCA24390.1621554777835693No Hit
GTATAGGGACTGAAGGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGACCCCCCAGGCTTGACCAGGCCTCCC23150.15391141085238336No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22660.15065367472635016No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22420.14905804886870128No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22410.1489915644579659No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20910.13901890284766028No Hit
GTATGGGAACTCAGCTCAACGCAGTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT20270.13476390056059653No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19700.13097428914868042No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19680.13084132032720966No Hit
CATCTATGGTGCGTCCAGCAGGGTCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA19630.13050889827353282No Hit
GTATTACTGTGCGAGAGAGAAAGTAGCTCGGGCCTTCCTTCTAGACTACTGGGGCCAGGGAACCCTGGTCACCGT19540.12991053857691448No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA19200.1276500686119119No Hit
GTCCCTATACCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAG19190.12758358420117652No Hit
GTAATACACTGCAGAATCTTGAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA18640.12392694161073112No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18600.12366100396778963No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTCTCTGCTGGTACCAGGCTAAGTAGTTGCTGCTAACACTCTGA18050.12000436137734424No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18010.11973842373440276No Hit
GTCTCCAGGCAGCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG17570.11681310966204644No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17360.11541693703660365No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT17260.11475209292924994No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17180.11422021764336698No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16810.11176029444615826No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16770.11149435680321677No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16600.11036412182071549No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG16410.10910091801674343No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16110.10710638569468231No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16000.10637505717659324No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT15820.10517833778335656No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15690.10431404044379675No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15590.10364919633644304No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTCCCTATACCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG15470.10285138340761857No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACC15450.10271841458614785No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTCAAGATT15100.10039146021040986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA1002.4630735E-644.16223570
GTGGTAT5150.042.7817271
TCTGGAG18700.041.32829370
GGTATCA15300.038.327181
GTATCAA37250.037.854651
TGGTATC6200.035.5198752
TATCAAC42700.033.2457352
GTCACCG20000.033.12167470
ATCAACG44200.032.171653
GCTTGTA1900.032.1122781
AATGGTA4650.030.0746470
CGCAGAG47450.030.027488
AGAGTAC47550.029.67766211
GCAGAGT49400.028.9097339
TAGTCAC605.58131E-428.60891238
ATAAACG500.00847947227.0854643
CAGAGTA53750.026.44413610
GAGTTTA7400.025.6512471
CGTTGTC18500.025.45130263
GCGTTGT18850.024.88928862
Produced by FastQC (version 0.11.5)