FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences941100
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATCTGTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCC37540.39889491021145473No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT30640.32557645308681327No Hit
GATATATATTGTTGCTGCCTGCAAATGAGCAGCAGTAATAATCAGCCTCA30290.32185740091382425No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC25960.27584741260227397No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA25610.2721283604292849No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC24180.2569333758367867No Hit
GATTATTACTGCTGCTCATTTGCAGGCAGCAACAATATATATCTGTTCGG23770.2525767718627139No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG23550.25023908192540645No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC22500.23908192540643924No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC21550.22898735522261185No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG20670.2196365954733822No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC20290.21559876739985123No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT18750.19923493783869942No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC18610.19774731696950376No Hit
GTAATATATAGCCGTGTCCTCGGGAGTCACAGAGTTCAGGTGCAGGGAGA18350.19498459249814049No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAACAGATATATATTGTTG17750.18860907448730208No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17230.1830836255445755No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16870.17925831473807247No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT16460.17490171076399957No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG16440.17468919349697162No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG16280.17298905536074805No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAACAGATATATATTGTTGCTGCCTGCAAATGAG15940.169376261821273No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAACAGATATATATTGTTGC15700.1668260546169376No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC15670.1665072787163957No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATTACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC15000.1593879502709595No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG13860.1472744660503666No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC13770.14631813834874083No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC13710.145680586547657No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATTACTATGTCTCCTGGTACCAACAGCA13640.1449367761130592No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATTT13520.1436616725108915No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG13330.14164275847412602No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG13310.14143024120709807No Hit
ATATATTACTGTGCAAGAGATGATAGTAGTGGCTGGTACAAATTGGACAA13150.13973010307087452No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG13020.13834874083519286No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC12240.13006056742110297No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA12190.12952927425353308No Hit
CCCCTGAGGGCCGCTTAGTGACGTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA11330.12039103177133142No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC11290.11996599723727552No Hit
GGATGAGGCTGATTATTACTGCTGCTCATTTGCAGGCAGCAACAATATATATCTGTTCGGAACTGGGACCAAGGT11050.11741579003294018No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT10580.11242163425778345No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10570.11231537562426948No Hit
GGTTATTACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGACGTCACTAAG10500.11157156518967166No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA10360.11008394432047604No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC10240.10880884071830836No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG10190.1082775475507385No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA10170.10806503028371055No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC10150.1078525130166826No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT10140.10774625438316862No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG10090.10721496121559877No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC10040.10668366804802891No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT10010.10636489214748698No Hit
GTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC9940.10562108171288918No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGG9900.10519604717883328No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC9850.10466475401126342No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9720.10328339177558177No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGAT300.00247541268.1353670
GGTATCA9850.048.575471
AGGGGGG2450.044.49656370
TCGTATG2100.044.3534940
CGTATGC2200.042.38517441
TCTCGTA2000.041.2858538
TGGTATC2200.040.3880422
ATCTCGT2100.039.2925337
TCTCCGA2450.039.04826711
GTATGCC2400.038.90321442
CACGAGA2600.038.1136621
GTATCAA27500.037.780361
TATACAC2850.037.172263
CCCACGA2600.036.8013519
CGAGACG2600.036.79939723
ATGCCGT2450.036.76899744
AGACGGA2550.036.19053325
CCGTCTT2350.035.51117347
GGAGCTA2550.034.8909429
GCCGTCT2400.034.7281746
Produced by FastQC (version 0.11.5)