FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences941100
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATCTGTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCC35840.38083094251407923No Hit
GATATATATTGTTGCTGCCTGCAAATGAGCAGCAGTAATAATCAGCCTCA31880.338752523642546No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT28000.29752417383912444No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC27880.29624907023695674No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27580.29306131123153756No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC23530.2500265646583785No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA23130.24577621931781957No Hit
GATTATTACTGCTGCTCATTTGCAGGCAGCAACAATATATATCTGTTCGG20900.22208054404420358No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG19910.21156093932632025No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG19650.20879821485495698No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC19190.2039103177133142No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC19090.2028477313781745No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC17560.1865901604505366No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT16930.17989586653915632No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG16930.17989586653915632No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16920.17978960790564233No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC16880.17936457337158646No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT16760.17808946976941878No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC16710.1775581766018489No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15830.16820741685261928No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAACAGATATATATTGTTG15410.1637445542450324No Hit
GTAATATATAGCCGTGTCCTCGGGAGTCACAGAGTTCAGGTGCAGGGAGA14930.15864413983636172No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAACAGATATATATTGTTGCTGCCTGCAAATGAG14750.1567314844331102No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATTACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC14490.1539687599617469No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAACAGATATATATTGTTGC13890.1475932419509085No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG13660.1451492933800871No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC13520.1436616725108915No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATTT13340.14174901710763999No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG13300.14132398257358408No Hit
ATATATTACTGTGCAAGAGATGATAGTAGTGGCTGGTACAAATTGGACAA12980.13792370630113698No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATTACTATGTCTCCTGGTACCAACAGCA12920.13728615450005313No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC12860.13664860269896928No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA12530.1331420677930082No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG12130.12889172245244926No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG11950.12697906704919776No Hit
CCCCTGAGGGCCGCTTAGTGACGTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA11920.12666029114865582No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG11050.11741579003294018No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC10870.11550313462968867No Hit
GGTTATTACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGACGTCACTAAG10810.11486558282860482No Hit
GGATGAGGCTGATTATTACTGCTGCTCATTTGCAGGCAGCAACAATATATATCTGTTCGGAACTGGGACCAAGGT10780.11454680692806292No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC10370.11019020295399001No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT10320.10965890978642014No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC10300.10944639251939221No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA9640.10243332270746999No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9600.10200828817341409No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT9510.10105196047178833No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC9470.10062692593773245No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTCG200.0071412452.14388346
GGTATCA10100.043.2798581
GTATCAA26600.039.6710851
ATAATAC655.944148E-736.7442473
TGGTATC3600.035.084212
TGCGTTG6950.034.86166470
TATCAAC31150.034.190782
CGCAGAG35300.030.3420018
AGAGTAC35450.029.82714711
GCAGAGT35850.029.7813559
CAGAGTA35950.029.60363210
ATCAACG36200.029.4069983
TCAACGC36250.029.2676494
AACGCAG37100.028.8698846
CAACGCA36950.028.802455
TCTCGCG3500.027.69012370
ACGCAGA38900.027.621697
AGACGGT17300.027.61009270
GACAGCG756.604742E-527.320411
CGATTAA1050.00965221526.37154870
Produced by FastQC (version 0.11.5)