FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1708971
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT47100.2756044426733982No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG46460.27185949907868534No Hit
GACTTAGGACGGTCAGCCTGGTCCCTCCGCCGAATAGCGCAAAATCGTTG43980.2573478426491731No Hit
TCTCTGGACTCCAGGCTGAGGATGAGGCTGATTATTTCTGCAGCTCATAT41200.2410807439096392No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC39500.23113323748618322No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT37480.21931325926537082No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCCTGGTCCCTC34240.20035448231713704No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCCTGGTCCCTCCGCCGAATAGCGCAAAATCGTTGCTGCCTCCATATGAG34010.1990086432127871No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC31930.18683757652997038No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC31330.1833266919099271No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC31110.18203936754924455No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG31090.18192233806190977No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT30850.18051798421389245No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCTTGGACAGTCAGTCACCATCTCCTG29930.17513462779649275No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT29860.17472502459082104No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG29580.17308661176813417No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG29300.1714481989454473No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCCGTGACATTGGTGGTTATAATTATGTCTCCTGGTATCAACAACA27420.16044742713597832No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26770.1566439687975981No Hit
GTCCAGAGACGGTCAGGGAGGCCGTGTTGCCATACTTGGACCCAGAGAAG26420.1545959527692395No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT26150.15301605469022003No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA25050.14657943288680733No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24770.14494102006412046No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24530.14353666621610314No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG24230.1417812239060815No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24070.1408449880074033No Hit
CATATGGAGGCAGCAACGATTTTGCGCTATTCGGCGGAGGGACCAGGCTGACCGTCCTAAGTCAGCCCAAGGCTG24040.14066944377640112No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC24030.14061092903273373No Hit
GGATGAGGCTGATTATTTCTGCAGCTCATATGGAGGCAGCAACGATTTTGCGCTATTCGGCGGAGGGACCAGG23920.13996726685239247No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG23770.13908954569738166No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCTTGGACAGTCAGTCAC23330.13651489697601657No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA23230.13592974953934267No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT23090.13511054312799925No Hit
CTCCTGCACTGGAACCAGCCGTGACATTGGTGGTTATAATTATGTCTCCTGGTATCAACAACACCCAGGCAAAGC22570.13206777645729506No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22480.13154114376428858No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG21330.12481194824253894No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20800.12171066682816736No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC20560.12030631298015004No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC20160.11796572323345453No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19740.11550810399942421No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA19690.11521553028108728No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA19380.11340157322739824No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC19240.11258236681605481No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18770.10983217386368756No Hit
CCCCTGAGGGCCGCTTAGTGACCTCAAAAATAATGAGTTGGGGGGCTTTGCCTGGGTGTTGTTGATACCAGGAGA18740.10965662963268541No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGC18700.10942257065801585No Hit
CTCTTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCCGTGACATT18500.10825227578466809No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCCTGGTC18340.10731603988598988No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC17680.10345406680394226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4100.061.1705670
GTGGTAT5500.041.2771871
CGTATGC5250.040.71441
TATACAC6000.040.659923
GACTAGG5550.040.3148526
TCGTATG5300.039.6098340
ACGAGAC5850.038.804222
CTTATAC5950.038.7248651
GATATAC350.001472967738.7237323
ATGCCGT5700.038.32369644
ATCTCGT4650.038.28652237
ACTAGGC5900.037.9432927
TCTCGTA4800.037.88288538
TAGGCAT5950.037.6741129
CTAGGCA6100.037.28042228
TGCCGTC5900.037.08011645
TTATACA6400.037.0598222
GTATGCC6000.036.84635542
GCCGTCT5950.036.2632146
GGATGGG10500.036.16969370
Produced by FastQC (version 0.11.5)