FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1708971
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57620.33716195301149054No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG46890.2743756330563831No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT43620.2552413118771471No Hit
GACTTAGGACGGTCAGCCTGGTCCCTCCGCCGAATAGCGCAAAATCGTTG40710.23821352146993716No Hit
TCTCTGGACTCCAGGCTGAGGATGAGGCTGATTATTTCTGCAGCTCATAT39860.2332397682582092No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37510.21948880349637298No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCCTGGTCCCTCCGCCGAATAGCGCAAAATCGTTGCTGCCTCCATATGAG33580.1964925092350894No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT33080.19356677205172002No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCCTGGTCCCTC32380.18947073999500283No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT31140.1822149117802467No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG30570.17887957139120558No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30160.17648046690084268No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC28810.16858097650574527No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCTTGGACAGTCAGTCACCATCTCCTG28580.16723513740139534No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC28490.16670850470838885No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28390.16612335727171498No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG26710.15629288033559377No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT25710.15044140596885494No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25670.1502073469941854No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25250.14774972776015508No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCCGTGACATTGGTGGTTATAATTATGTCTCCTGGTATCAACAACA24380.1426589450610923No Hit
GTCCAGAGACGGTCAGGGAGGCCGTGTTGCCATACTTGGACCCAGAGAAG24220.1417227091624141No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCTTGGACAGTCAGTCAC23920.13996726685239247No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA23640.1383288540297056No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23570.13791925082403386No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG23040.13481796940966229No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22850.13370618927998193No Hit
GGATGAGGCTGATTATTTCTGCAGCTCATATGGAGGCAGCAACGATTTTGCGCTATTCGGCGGAGGGACCAGG22790.1333551008179776No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22460.1314241142769538No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22430.13124857004595164No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG22160.12966867196693216No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT22100.1293175835049278No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG21790.1275036264512388No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21180.1239342270875281No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC20920.12241284375217602No Hit
CTCCTGCACTGGAACCAGCCGTGACATTGGTGGTTATAATTATGTCTCCTGGTATCAACAACACCCAGGCAAAGC20630.12071591618582175No Hit
CATATGGAGGCAGCAACGATTTTGCGCTATTCGGCGGAGGGACCAGGCTGACCGTCCTAAGTCAGCCCAAGGCTG20600.1205403719548196No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20190.11814126746445668No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC20130.11779017900245235No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC19840.11609325143609811No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC19040.11141207194270704No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA18840.11024177706935928No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGC18780.10989068860735496No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18510.10831079052833548No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18140.10614574501264212No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18020.10544356808863345No Hit
CCCCTGAGGGCCGCTTAGTGACCTCAAAAATAATGAGTTGGGGGGCTTTGCCTGGGTGTTGTTGATACCAGGAGA17310.1012890212882489No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCCTGGTC17220.1007623885952424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6400.042.339751
TCAACGC57000.033.365134
ATCAACG57600.033.203553
AACGCAG58750.032.5431256
CAACGCA58600.032.511895
ACGCAGA61150.031.2658827
CGCAGAG61450.030.9480388
TCGGTTT950.004462525730.8873270
AATGGTA5550.030.40035670
AGAGTAC62550.030.2414911
GTACTAG450.00505807130.1082671
CAGAGTA62900.030.0185410
GGATGGG10200.029.48679270
AAAACCG2903.45608E-1027.82521270
GCAGAGT69600.027.1782369
GTATCAA75100.026.384821
GTCGGTT859.3196484E-426.20565669
TCCGAGA6500.025.95722870
AGTACGG54500.025.64845513
GAGTACG54800.025.56606512
Produced by FastQC (version 0.11.5)