FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314411
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40110.3051556933105398No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37030.28172314443503593No Hit
CAGTAATACACGGCCGTGTCGTCAAATCGCAGGTTGCTCAGTTCCATGTA32250.24535704585552007No Hit
GGTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA27210.20701287496833182No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26610.20244809271985706No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26150.19894842632935966No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24750.1882972677495852No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23750.18068929733546052No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23530.1790155438443531No Hit
CATTTATGGTGCATCCAGCAGGGCCGCTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGCGACAGACTCCA21990.16729926940660114No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21620.164484320353375No Hit
GCTCAGGTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT21610.16440824064923376No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21450.1631909653829738No Hit
CTCCTGCAGGGCCAGTCCGAGTGTTAGCAACAGTTTCTTAGCCTGGTACC21030.15999561780904145No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20810.15832186431793405No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20370.15497435733571918No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20300.15444179940673045No Hit
GTAATACACGGCCGTGTCGTCAAATCGCAGGTTGCTCAGTTCCATGTAGG20210.15375708206945923No Hit
GCATTATGGTAGCTCACCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGG20000.15215940828249308No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19320.1469859884008883No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18990.14447535816422716No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18810.14310592348968473No Hit
CCATAAATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGAAACTGTTGCTAACACTCGGA18800.14302984378554348No Hit
GTCCGAGTGTTAGCAACAGTTTCTTAGCCTGGTACCAGCAGAAACCTGGC18770.14280160467311975No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18570.14128001059029482No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17750.1350414748507126No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCCGAGTGTTAGCAA17640.1342045981051589No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17610.13397635899273513No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17600.13390027928859388No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCCGA17420.13253084461405146No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17200.13085709112294405No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC16900.12857469999870663No Hit
GTATTACTGTGCGTGGGGGTGGGGGGGAGGACCCCTGGACTACTGGGGCC16820.12796606236557667No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGAGGTGAGCTACCATAATG16100.12248832366740692No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT15970.12149928751357073No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC15620.11883649786862709No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15270.11617370822368346No Hit
GTTCCATGTAGGCTGTGGTCGTGGATTCGTCCGCGGTAATCGTGACTCTG15050.11449995473257603No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCACCAC14910.11343483887459858No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAGCTACAGGTGTCCAGTCCCAGGTGCA14760.11229364331247987No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14600.11107636804621995No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14410.10963085366753626No Hit
GTGTATTACTGTGCGTGGGGGTGGGGGGGAGGACCCCTGGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC14290.1087178972178413No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14040.10681590461431015No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14000.10651158579774514No Hit
ACCATAATGCTGACAATAGTAAACTGCAAAATCTTCAGGCTCCAGTCTGC13830.10521823082734395No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13560.1031640788155303No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13510.10278368029482407No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13400.10194680354927035No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13400.10194680354927035No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13380.10179464414098788No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13320.1013381659161404No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)