FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences972251
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGGTTCAGGGAGA34770.35762370005276417No Hit
GTATTACTGTCAGCAGTATGGTAGCTCACCTCCCACTTTCGGCGGAGGGA25750.26484930331776463No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25010.25723810003795317No Hit
GTGTATTACTGTGCGAGACATTTCTGGATCATCGATAGTGCCTACGCAGGGGGTCGTAACTGGTACTTCAATCT23560.24232425577345768No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG22260.2289532229845997No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21080.21681643937625159No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCG20840.2143479410152317No Hit
CTATTATACTGGGAGCACTTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATCTCCGTAGACACGTCCAAGAA20370.20951379839156758No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA18560.1908972065855422No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA17250.17742331969830835No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGGTTCAGGGA16630.17104636559900685No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA16520.1699149705168727No Hit
GTATAATAGAAAGTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCCCCAGTAGTAACTACTA15520.1596295606792896No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG13610.1399844278895059No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC13390.13772163772523763No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13390.13772163772523763No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA13080.13453316067558685No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13070.13443030657721103No Hit
GTATTACTGTGCGAGACATTTCTGGATCATCGATAGTGCCTACGCAGGGG12750.13113897542918443No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12350.1270248114941512No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGG12280.12630483280552038No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12090.12435060493637959No Hit
GTATGGTAGCTCACCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT11880.12219066887048714No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA11310.11632798526306479No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10980.11293380001666235No Hit
GGGTGCCACGGCCCCAGAGATTGAAGTACCAGTTACGACCCCCTGCGTAGGCACTATCGATGATCCAGAAATGT10580.10881963608162912No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT10470.10768824099949498No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT10420.10717397050761585No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACC10400.10696826231086418No Hit
GCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGGTTG10310.10604257542548168No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTT10230.10521974263847504No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC10050.10336836886771009No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC9970.10254553608070344No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT9880.10161984919532095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8700.044.412431
TACGGAG14600.043.9118870
GTATCAA23700.039.5318831
AGTTTAT800.002342833735.22601770
TATCAAC27700.033.5591432
AATGGTA3650.032.81327470
ATCAACG28700.032.37653
TCAACGC29000.031.9106334
CAACGCA29350.031.5268525
AACGCAG29400.031.4732346
TGGTTCG450.004537631830.78279543
ACGCAGA30850.030.1046247
CTACGGA15450.029.85711969
AGAGTAC31300.029.77936411
CGCAGAG31550.029.5449128
GCAGAGT32100.028.9323239
GCTTGTA1202.9522198E-928.49471
GTACAAT500.00807836927.354911
AACTGTG14200.027.28775870
CAGAGTA34650.026.80310410
Produced by FastQC (version 0.11.5)