FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1211315
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCGTCGCCTCGCAGGCTGTTCATTTGCAAATACA45350.37438651383001115No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42400.3500328155764603No Hit
GTATTATTGTCAGTGTTATGGTAGCTCACCTCAACGCAATTTTGGCCAGGGGACCAGGCTGCAGATCACACGAA37200.30710426272274344No Hit
GTGTATTACTGTGCGAGAGAGAGAGCAGCTCGGGCCTTCCTCCTAGACAA28770.23751047415412177No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28680.23676747997011513No Hit
GTGTTATGGTAGCTCACCTCAACGCAATTTTGGCCAGGGGACCAGGCTGC26300.21711941154860626No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA24270.20036076495378988No Hit
GTCCCTATACCATGAACTGGGTCCGCCAGGGTCCAGGGAAGGGGCTGGAG23920.1974713431270974No Hit
CAATAATACATTGCGAAATCTTCAGGGTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA22850.18863796782835185No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18650.15396490590804207No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18150.14983716044133855No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17660.14579196988396909No Hit
CAGTAATACACAGCCGTGTCGTCGCCTCGCAGGCTGTTCATTTGCAAATA17340.1431502127852788No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17050.14075612041459076No Hit
GTCTCCAGGCATCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAA16050.13250062948118368No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15630.12903332328915268No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15540.12829032910514607No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAACAACAACTTAGCCTGGTACC15340.12663923091846466No Hit
TACATAGACTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCGCTGTATTTG15110.12474046800378101No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15030.12408002872910844No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGACCCTGAAGATT14900.12300681490776554No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14580.12036505780907526No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA14380.11871395962239385No Hit
ACCATAACACTGACAATAATACATTGCGAAATCTTCAGGGTCCAGTCTGC13720.11326533560634516No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGCT13560.11194445705700004No Hit
GTATTACTGTGCGAGAGAGAGAGCAGCTCGGGCCTTCCTCCTAGACAACTGGGGCCGGGGAACCCTGGTCACCGT13410.11070613341698898No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTGTTGTTGCTAACACTCTGA13300.1097980294143142No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG12980.10715627231562394No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12950.10690860758762172No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12790.10558772903827658No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12760.10534006431027437No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12660.10451451521693367No Hit
GCCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGC12330.10179020320890933No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12290.10145998357157304No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12220.10088209920623453No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12180.10055187956889826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG15050.045.22762370
TCGACTC350.001436828938.9190520
GGTATCA13400.038.9116861
GTATCAA34950.038.9062231
TATCAAC38100.035.2117582
CTCGCCG400.00276288134.0654324
ATCAACG40750.032.9932063
AACGCAG41200.032.868476
GTCACCG13450.031.30383370
CGCAGAG44300.030.5684178
AGAGTAC45300.030.11770211
TTGCGCG803.061361E-629.79124318
CGTTGTC14500.029.63371563
GCAGAGT45800.029.5672689
GCGTTGT14750.029.55277362
GGCGTTG15050.029.13162861
ACAGGTA2500.028.6267931
TTGGCGT15200.028.23592459
TGGCGTT15650.028.16213660
CGAGTTC16000.028.06193452
Produced by FastQC (version 0.11.5)