FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423151
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14090.33297806220474485No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12460.2944575340717616No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12320.29114902245297775No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11460.27082530822330564No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCACCGAATAACACACCGTTCCTGCCTGCATATGAGCTG10870.2568822949727166No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC10560.24955630495969525No Hit
CATTATTACTGCAGCTCATATGCAGGCAGGAACGGTGTGTTATTCGGTGGAGGGACCAAACTGACCGTCCTAGGT10440.24672043785788053No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGCCAGTCAGTCACCATCTCCTG10090.2384491588109209No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCACCGAATAACACACCGTTCCTG9950.2351406471921371No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9450.22332453426790908No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC9230.21812544458124877No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9140.21599854425488774No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9110.21528957747943403No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG8620.20370978681369065No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG8590.20300082003823694No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7870.18598561742734862No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7860.18574929516886407No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7630.1803138832237192No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTACAACTCTGTCTCCTGGTACCAACAGCACCCAGGCAGAGC7610.1798412387067501No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT7560.17865962741432725No Hit
GTGTTATTCGGTGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG7260.17156995965979047No Hit
GTAATAATGAGCCTCATCCTCAGCCAGGAGCCCAGAGACGGTCAGGGAGG7120.16826144804100662No Hit
TCTCTGGGCTCCTGGCTGAGGATGAGGCTCATTATTACTGCAGCTCATAT7110.16802512578252207No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGCCAGTCAGTCAC7090.16755248126555294No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG7080.1673161590070684No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC6980.1649529364222228No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG6890.16282603609586177No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA6890.16282603609586177No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG6450.1524278567225411No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA6440.15219153446405656No Hit
GAATAACACACCGTTCCTGCCTGCATATGAGCTGCAGTAATAATGAGCCT6290.14864670058678817No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA6150.14533818896800432No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA6120.14462922219255064No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC5920.13990277702285947No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC5780.13659426540407563No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT5440.12855930861560058No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG5330.12595976377227042No Hit
GTAATACACAGCCGTGTCTTCGGGAGTCACAGAGTTCAGCTGCAGGGAGA5270.12454183022136305No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT5250.12406918570439394No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5230.12359654118742482No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT5220.12336021892894027No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC5160.1219422853780329No Hit
GGATGAGGCTCATTATTACTGCAGCTCATATGCAGGCAGGAACGGTGTGTTATTCGGTGGAGGGACCAAACTGAC5090.12028802956864097No Hit
GTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTACAACTC5070.11981538505167187No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCACCGAATAACACAC5000.11816112924227995No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCACCGAATAACACACCGTTC4990.1179248069837954No Hit
TTATTACTGCAGCTCATATGCAGGCAGGAACGGTGTGTTATTCGGTGGAG4970.11745216246682626No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT4840.11437997310652698No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGCCAGTCAGTCACCATCT4810.11367100633107331No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC4750.11225307278016596No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA4720.11154410600471226No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC4710.11130778374622771No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTACAACTCTGTCTCCTGGTACCAACAGCA4680.11059881697077403No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG4630.10941720567835121No Hit
GTTTGGTCCCTCCACCGAATAACACACCGTTCCTGCCTGCATATGAGCTGCAGTAATAATGAGCCTCATCCTCAG4610.10894456116138211No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGCCAGTCAGTCACCATCTCCTGCA4460.10539972728411372No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG4450.10516340502562914No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT4430.10469076050866002No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT4400.10398179373320635No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC4330.10232753792381442No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGCCAGTCAGTCAC4300.10161857114836075No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT4290.1013822488898762No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC4280.10114592663139163No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG4280.10114592663139163No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC4250.10043695985593795No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATT252.7465165E-454.44253517
CGACCAA200.0069835952.43390349
TGGGTTG2250.043.68100470
GGTATCA5550.042.9214861
TCGCTGC405.417278E-542.5382582
AGGGGGG1853.6379788E-1241.74149370
GTATCAA14650.040.8831861
CGCAATC458.070412E-539.75687462
GTGGTAT1600.038.284431
TGCACCG451.0852884E-437.8073166
CTCTTAT1100.037.12431
ATGCCGT1900.036.54728344
CGTATGC2000.036.2852941
TGGTATC1700.036.032412
TATCAAC16500.035.8868262
GCTAATC1550.035.3203733
TAGGGAT501.7005015E-435.0282250
ACACTCG2150.034.86734427
TCGTATG2100.034.52841640
TCTCGTA1600.034.43757638
Produced by FastQC (version 0.11.5)