FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004686328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423151
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20690.4889507528045544No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13470.3183260821787022No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11970.2828777434060182No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11040.2608997733669541No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10560.24955630495969525No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGCCAGTCAGTCACCATCTCCTG10210.24128502591273565No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCACCGAATAACACACCGTTCCTGCCTGCATATGAGCTG9860.23301374686577606No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9580.22639672362820837No Hit
CATTATTACTGCAGCTCATATGCAGGCAGGAACGGTGTGTTATTCGGTGGAGGGACCAAACTGACCGTCCTAGGT9530.22521511233578556No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG9340.22072498942457894No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCACCGAATAACACACCGTTCCTG8860.20938152101732005No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC8710.20583668714005166No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGCCAGTCAGTCAC8540.20181920874581413No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC8430.199219663902484No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8410.19874701938551487No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG7890.18645826194431775No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT7760.18338607258401848No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTACAACTCTGTCTCCTGGTACCAACAGCACCCAGGCAGAGC7420.17535111579554344No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7420.17535111579554344No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG7160.16920673707494488No Hit
GTGTTATTCGGTGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG7130.1684977702994912No Hit
TCTCTGGGCTCCTGGCTGAGGATGAGGCTCATTATTACTGCAGCTCATAT7050.16660719223161471No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC6910.16329868061283087No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG6680.15786326866768602No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG6250.14770141155284994No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA6120.14462922219255064No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA6110.1443928999340661No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG5850.13824852121346753No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA5780.13659426540407563No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC5690.13446736507771456No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC5570.13163149797589985No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC5290.1250144747383322No Hit
GAATAACACACCGTTCCTGCCTGCATATGAGCTGCAGTAATAATGAGCCT5180.12241492989500202No Hit
GTAATAATGAGCCTCATCCTCAGCCAGGAGCCCAGAGACGGTCAGGGAGG4950.11697951794985713No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA4880.1153252621404652No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC4880.1153252621404652No Hit
GTGTGTTATTCGGTGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC4860.1148526176234961No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC4800.11343468407258873No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCACCGAATAACACAC4770.11272571729713506No Hit
GTAATACACAGCCGTGTCTTCGGGAGTCACAGAGTTCAGCTGCAGGGAGA4760.1124893950386505No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG4760.1124893950386505No Hit
TTATTACTGCAGCTCATATGCAGGCAGGAACGGTGTGTTATTCGGTGGAG4680.11059881697077403No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA4660.1101261724538049No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT4570.10799927212744387No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCACCGAATAACACACCGTTC4560.10776294986895932No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGCCAGTCAGTCAC4550.10752662761047474No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT4540.10729030535199019No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT4510.10658133857653651No Hit
GTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTACAACTC4510.10658133857653651No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC4500.10634501631805195No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGCCAGTCAGTCACCATCT4420.10445443825017547No Hit
GGATGAGGCTCATTATTACTGCAGCTCATATGCAGGCAGGAACGGTGTGTTATTCGGTGGAGGGACCAAACTGAC4330.10232753792381442No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT4270.10090960437290707No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)