FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1466549
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACAGCCCCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA98450.6713038568776086No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT71290.4861071808715563No Hit
GTATTGGTACCTGCAGAAGCCAGGCCAGCCTCCGCAGCTCCTGATGTATG62790.42814798550883737No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC61830.4216020057972833No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC48520.33084472458813174No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG44710.3048653676079013No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44000.30002407011289767No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG43430.29613739465916245No Hit
GTGATGGAAGGACCTATCTGTATTGGTACCTGCAGAAGCCAGGCCAGCCTCCGCAGCTCCTGATGTATGAAGTTT41130.2804543182668973No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37750.25740701469913385No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA37240.2539294629773707No Hit
CCCCTGGACAGTCGGCCTCCATCTCCTGTAAGTCTAGTCAGAGCCTCCTG36330.24772441970912665No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36050.24581517562659005No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG36000.24547423918327996No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG34370.23435971113137033No Hit
CCCCTGGACAGTCGGCCTCCATCTCCTGTAAGTCTAGTCAGAGCCTCCTGCATAGTGATGGAAGGACCTAT34080.23238227976017164No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33030.22522261445065933No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT32510.22167687544023418No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATCCAGTGCAGATATTGTGATGA32460.22133593899692408No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG31630.21567639403797623No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31630.21567639403797623No Hit
GGATACCTGGATCCAGTGCAGATATTGTGATGACCCAGACTCCACTCTCTCTGTCCGTCACCCCTGGACAGTCGG30080.20510736429536278No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC29190.19903869560444284No Hit
GTCCAGGGGTGACGGACAGAGAGAGTGGAGTCTGGGTCATCACAATATCT28830.19658395321261002No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG28200.1922881540269026No Hit
GATCAGGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATA27690.1888106023051395No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27250.18581036160401052No Hit
GCTGTATACTTTGCATACAATAATAAACCCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGCGTGAAAT26540.18096906410900693No Hit
GGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG26410.18008262935640063No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTTCTCAGTTCATCTTCTCACC26410.18008262935640063No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGAGGGGG25020.17060459623237956No Hit
GCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATCCAGTGCAGATATTGTGATGACCCAGACTCCACTCTCT24200.1650132385620937No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24200.1650132385620937No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24050.1639904292321634No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23670.16139931226300655No Hit
GGTCAGGGACAGATTTCACGCTGAAAATCAGCCGGGTGGAGGCTGAGGAT23610.16099018853103442No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23470.1600355664897661No Hit
CCTGGATCCAGTGCAGATATTGTGATGACCCAGACTCCACTCTCTCTGTC23380.15942188089180792No Hit
CTCTCTGTCCGTCACCCCTGGACAGTCGGCCTCCATCTCCTGTAAGTCTA23140.1577853859639194No Hit
TTATTATTGTATGCAAAGTATACAGCCCCCTCTCACTTTCGGCGGAGGGA23100.15751263680927127No Hit
GCTAATGCTCTGGATACCTGGATCCAGTGCAGATATTGTGATGACCCAGACTCCACTCTCTCTGTCCGTCACCC22980.15669438934532703No Hit
GTACCAATACAGATAGGTCCTTCCATCACTATGCAGGAGGCTCTGACTAGACTTACAGGAGATGGAGGCCGA22790.1553988308607486No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG22680.15464877068546637No Hit
CTCCTGCATAGTGATGGAAGGACCTATCTGTATTGGTACCTGCAGAAGCC22480.15328502491222593No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22120.15083028252039313No Hit
ATCTGGCACTCCAGAGAGCCGGTTGGAAACTTCATACATCAGGAGCTGCGGAGGCTGGCCTGGCTTCTGCAGGTA22030.15021659692243491No Hit
GTGCAGATATTGTGATGACCCAGACTCCACTCTCTCTGTCCGTCACCCCT21410.14598898502538954No Hit
CATCAGGAGCTGCGGAGGCTGGCCTGGCTTCTGCAGGTACCAATACAGAT21380.1457844231594035No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC21260.1449661756954592No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20960.14292055703559853No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20950.14285236974693652No Hit
ATGCAGGAGGCTCTGACTAGACTTACAGGAGATGGAGGCCGACTGTCCAGGGGTGACGGACAGAGAGAGTGGAGT20740.14142043668503407No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT20730.14135224939637203No Hit
GTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT20410.13917025615918732No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATCCAGTGCAGATATTGTGATGACCCAGACTCCACT20400.1391020688705253No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA20250.13807925954059497No Hit
GTATACTTTGCATACAATAATAAACCCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGCGTGAAATCTGTCC20040.1366473264786925No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC20040.1366473264786925No Hit
TTCCATCACTATGCAGGAGGCTCTGACTAGACTTACAGGAGATGGAGGCC19840.13528358070545204No Hit
GCTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGTCAGGGACAGATT19680.1341925840868597No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19510.13303340017960533No Hit
CCAGAGAGCCGGTTGGAAACTTCATACATCAGGAGCTGCGGAGGCTGGCC19230.13112415609706868No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT19160.13064684507643454No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19050.1298967849011523No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18900.12887397557122196No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT18790.12812391539593973No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18720.12764660437530556No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA18700.12751022979798152No Hit
CAATAATAAACCCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGCGTGAAATCTGTCCCTGACCCGCTGCCA18650.12716929335467142No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC18250.12444180180819053No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18230.12430542723086647No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC18090.12335080518959815No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT17960.12246437043699188No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17850.12171431026170965No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17750.12103243737508942No Hit
CCTGCATAGTGATGGAAGGACCTATCTGTATTGGTACCTGCAGAAGCCAG17470.11912319329255279No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC17420.11878225684924268No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATCCAGTGCAGATATTGTGATGACCCAGACTC16240.11073615678712405No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16220.11059978220980002No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16190.11039522034381394No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16120.1099179093231798No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC16050.10944059830254563No Hit
AGCCAGGCCAGCCTCCGCAGCTCCTGATGTATGAAGTTTCCAACCGGCTC16040.1093724110138836No Hit
GCATAGTGATGGAAGGACCTATCTGTATTGGTACCTGCAGAAGCCAGGCC15490.10562211013747239No Hit
CCACTCTCTCTGTCCGTCACCCCTGGACAGTCGGCCTCCATCTCCTGTAA15370.10480386267352813No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT15210.10371286605493577No Hit
GTTCAGTGGCAGCGGGTCAGGGACAGATTTCACGCTGAAAATCAGCCGGG15170.10344011690028769No Hit
GTGCCAGATAGGTTCAGTGGCAGCGGGTCAGGGACAGATTTCACGCTGAA15150.10330374232296363No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT15100.10296280587965352No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15050.10262186943634341No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC15040.10255368214768139No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15010.10234912028169532No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG15010.10234912028169532No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG14970.10207637112704725No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14920.10173543468373714No Hit
CTTCATACATCAGGAGCTGCGGAGGCTGGCCTGGCTTCTGCAGGTACCAA14870.10139449824042703No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14860.10132631095176499No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT401.0310884E-466.37945670
GTACGTA551.4822508E-647.54652869
GGTATCA15100.043.1083031
GGTACGT605.871072E-638.92341668
GTATCAA39100.038.5256541
GTGGTAT4350.038.38941
TGGTATC4450.038.292572
GTCGTAT557.433813E-637.60819639
CGAGACG4700.035.5500123
TCGTATG2850.035.12260440
GACGTAG4600.034.86049326
ACGAGAC4800.034.80582422
CGTATGC2800.034.55403541
ACGTAGA4650.034.49978327
AGACGTA4650.034.47387725
ATCTCGT2500.034.38286237
GACAGGT2300.034.0803871
TCTATAC400.002757131134.0803873
ATGCCGT2850.034.0774244
CGTAGAG4700.033.42251228
Produced by FastQC (version 0.11.5)