FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1466549
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACAGCCCCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA89930.6132082869375657No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT69650.47492446553098466No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC47200.32184400248474476No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46380.31625264481445897No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC45880.3128432803813579No Hit
GTATTGGTACCTGCAGAAGCCAGGCCAGCCTCCGCAGCTCCTGATGTATG43060.2936144649786676No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40090.273362840246047No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG39210.26736235884378906No Hit
GCTGTATACTTTGCATACAATAATAAACCCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGCGTGAAAT39040.26620317493653467No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37450.25536139603927316No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33550.22876835346108448No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT33240.2266545475125618No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG31820.21697195252255463No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31600.21547183217199015No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT31460.21451721013072186No Hit
CCCCTGGACAGTCGGCCTCCATCTCCTGTAAGTCTAGTCAGAGCCTCCTGCATAGTGATGGAAGGACCTAT30510.20803941770782974No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC29350.20012969222303517No Hit
GTGATGGAAGGACCTATCTGTATTGGTACCTGCAGAAGCCAGGCCAGCCTCCGCAGCTCCTGATGTATGAAGTTT29240.19937963204775294No Hit
CAATAATAAACCCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGCGTGAAATCTGTCCCTGACCCGCTGCCA29070.19822044814049852No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29030.19794769898585046No Hit
GTATACTTTGCATACAATAATAAACCCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGCGTGAAATCTGTCC28610.1950838328620455No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28510.19440195997542528No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28290.19290183962486082No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28150.1919472175835925No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATCCAGTGCAGATATTGTGATGA27540.18778779297520914No Hit
GTCCAGGGGTGACGGACAGAGAGAGTGGAGTCTGGGTCATCACAATATCT27070.1845829904080941No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26830.18294649548020556No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG26360.17974169291309053No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT25590.1744912716861148No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTTCTCAGTTCATCTTCTCACC25220.17196834200562No Hit
GATCAGGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATA25180.1716955928509719No Hit
GGATACCTGGATCCAGTGCAGATATTGTGATGACCCAGACTCCACTCTCTCTGTCCGTCACCCCTGGACAGTCGG24730.1686271648611809No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGAGGGGG24140.1646041148301216No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24090.16426317838681148No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23970.16344493092286722No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23500.16024012835575216No Hit
GTCCCTGACCCGCTGCCACTGAACCTATCTGGCACTCCAGAGAGCCGGTT23340.15914913173715983No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA22400.15273952660292972No Hit
GGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG21850.14898922572651851No Hit
TTATTATTGTATGCAAAGTATACAGCCCCCTCTCACTTTCGGCGGAGGGA21550.14694360706665785No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21360.14564804858207941No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21210.1446252392521491No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20790.14176137312834416No Hit
GCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATCCAGTGCAGATATTGTGATGACCCAGACTCCACTCTCT20630.1406703765097518No Hit
CCCCTGGACAGTCGGCCTCCATCTCCTGTAAGTCTAGTCAGAGCCTCCTG20360.1388293197158772No Hit
ATAATAAACCCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGCGTGAAATCTGTCCCTGACCCGCTGCCA20170.1375337612312988No Hit
GTACCAATACAGATAGGTCCTTCCATCACTATGCAGGAGGCTCTGACTAGACTTACAGGAGATGGAGGCCGA19950.1360336408807343No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19680.1341925840868597No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19520.13310158746826734No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19510.13303340017960533No Hit
ATCTGGCACTCCAGAGAGCCGGTTGGAAACTTCATACATCAGGAGCTGCGGAGGCTGGCCTGGCTTCTGCAGGTA19460.1326924637362952No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19270.1313969052517168No Hit
GGTCAGGGACAGATTTCACGCTGAAAATCAGCCGGGTGGAGGCTGAGGAT19200.13091959423108263No Hit
CATCAGGAGCTGCGGAGGCTGGCCTGGCTTCTGCAGGTACCAATACAGAT19100.1302377213444624No Hit
CCTGGATCCAGTGCAGATATTGTGATGACCCAGACTCCACTCTCTCTGTC18640.12710110606600938No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18530.12635104589072713No Hit
GTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT18460.12587373487009298No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18370.1252600492721348No Hit
ATGCAGGAGGCTCTGACTAGACTTACAGGAGATGGAGGCCGACTGTCCAGGGGTGACGGACAGAGAGAGTGGAGT18150.12375992892157031No Hit
GCTAATGCTCTGGATACCTGGATCCAGTGCAGATATTGTGATGACCCAGACTCCACTCTCTCTGTCCGTCACCC18080.12328261790093614No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATCCAGTGCAGATATTGTGATGACCCAGACTCCACT17950.12239618314832987No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17920.12219162128234379No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17640.12028237719980715No Hit
GTGCAGATATTGTGATGACCCAGACTCCACTCTCTCTGTCCGTCACCCCT17500.11932775515853887No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17410.11871406956058066No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17350.11830494582860854No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC17330.11816857125128448No Hit
TTCCATCACTATGCAGGAGGCTCTGACTAGACTTACAGGAGATGGAGGCC17100.11660026361205796No Hit
GGTGGAGGCTGAGGATGTTGGGGTTTATTATTGTATGCAAAGTATACAGCCCCCTCTCACTTTCGGCGGAGGGAC16860.11496376868416944No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16830.11475920681818337No Hit
TCCTCAGCCTCCACCCGGCTGATTTTCAGCGTGAAATCTGTCCCTGACCCGCTGCCACTGAACCTATCTGGCACT16730.11407733393156315No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT16650.11353183562226696No Hit
CTCTCTGTCCGTCACCCCTGGACAGTCGGCCTCCATCTCCTGTAAGTCTA16530.1127135881583227No Hit
GGCTGTATACTTTGCATACAATAATAAACCCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGCGTGAAAT16490.1124408390036746No Hit
CTCCTGCATAGTGATGGAAGGACCTATCTGTATTGGTACCTGCAGAAGCC16450.11216808984902651No Hit
CCAGAGAGCCGGTTGGAAACTTCATACATCAGGAGCTGCGGAGGCTGGCC16390.11175896611705438No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16290.11107709323043416No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16150.11012247118916585No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15720.10719041777669891No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15140.10323555503430161No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15030.10248549485901938No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14820.10105356179711691No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG14810.10098537450845488No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)