FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495249
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATATACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGAGA56700.3792010561451638No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44590.2982112009437893No Hit
GTATTACTGTCAGCAGTATGGTAGCTCAGTGGGGACGTTCGGCCAAGGGA42400.2835648109445317No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39510.2642369264249633No Hit
GTATATCACTGTGCGAGCGCGGACTTGCCTAAATACGATTTTTGGAGTGGTCCCACGGCATTTGACGACTGGGGC38990.26075924478130397No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA31130.20819274916752997No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30540.20424691807183953No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30270.20244119875686256No Hit
CAGTAATACACTGCAGAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACCGCCA29680.19849536766117212No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29220.19541895697639658No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAATTACTACTGGGGCTGGATCCG29060.19434890108603986No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCAGTGGGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAA27940.1868585098535428No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26130.17475350259388234No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT25350.16953698012839333No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCGGTGGGTCTGGGACAGACTTCA25080.16773126081341636No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23980.16037462656721388No Hit
GTAATACACTGCAGAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACCGCCA23000.15382053423877895No Hit
GTCCCAGACCCACCGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT21480.14365500328039008No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21150.14144801300652934No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20480.13696715396566056No Hit
CCATAGATGAGGGGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCTGCTAACACTCTGA20080.13429201423976878No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19670.13154999602072967No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19550.13074745410296212No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19390.12967739821260538No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19300.12907549177427974No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18980.12693537999356627No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18780.12559781013062038No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18580.12426024026767447No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18510.12379209081564342No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18360.12278891341843398No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18350.12272203492528669No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTAATTACTACTGGG18060.12078255862401513No Hit
CTCCAGGCACCTTGTCTTTGTCTCCAGGGGAAAGAGTCACCCTCTCGTGC18060.12078255862401513No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17920.119846259719953No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17760.11877620382959628No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17550.11737175547350308No Hit
GTATGGTAGCTCAGTGGGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT17290.11563291465167341No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17140.11462973725446397No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16460.1100819997204479No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16330.10921257930953306No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16270.10881130835064928No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15840.1059355331453156No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15810.1057348976658737No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAT15560.10406293533719133No Hit
GTCTCCAGGCACCTTGTCTTTGTCTCCAGGGGAAAGAGTCACCCTCTCGTGCAGGGCCAGTCAGAGTGTTAGCAG15500.10366166437830757No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTC15300.10232409451536165No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15160.10138779561129953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3350.048.8518181
CGGATAG450.00993962447.95773370
TACGGAT19150.046.95670
ACTATCG405.3950054E-542.5767828
GTATCAA29650.039.0965581
GGTATCA13750.036.4501151
GTGCGGA1201.0085072E-535.968370
TTGAACG502.0026835E-434.0739633
ATTCCGT400.002764680434.0614246
GAGTTTA5450.033.1561161
ATCAACG35900.032.4604343
TATCAAC35850.032.319942
GATTCAC1600.031.963591
TCAACGC36450.031.9631484
CAACGCA36950.031.526415
CTCGCTA652.5208674E-531.46552323
AACGCAG37450.031.3783516
ACGCAGA38700.030.364847
TATAGCT450.004903170330.30013723
CGCAGAG38800.030.286588
Produced by FastQC (version 0.11.5)