FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1480700
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47530.32099682582562306No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41170.2780441682987776No Hit
GAGTTACATGGTCCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT37930.2561626257851017No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31730.21429053825893157No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG31290.21131897075707434No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30800.2080097251300061No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG29400.1985547376240967No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCGTCAAGATTCAGTGGCAGTGGGTCTGGGACACACTTCA27560.18612818261633013No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26860.18140068886337543No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26810.1810630107381644No Hit
CACTTCACTCTCACCATCAGCGGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAGCAGAGTTACATGGTC26650.17998244073748904No Hit
ATGTAACTCTGCTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAAGTGTGTC26340.17788883636118052No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26080.17613291011008306No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25340.17113527385695954No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24820.16762342135476463No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24410.16485446072803403No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24390.16471938947794962No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAGGTCAGAGCGTTAGCAACTATTTAAATTGGTATCAGCAGAAA23720.16019450260012158No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA23480.15857364759910852No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG22500.15195515634497198No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22330.1508070507192544No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21860.14763287634227054No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21810.1472951982170595No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGCCTTTTT21660.14628216384142634No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAGGTCAGAGCGTTAGCAA21250.14351320321469577No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG20980.14168974133855608No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20890.1410819207131762No Hit
GGTCAGAGCGTTAGCAACTATTTAAATTGGTATCAGCAGAAACCAGGGACAGCCCCTAAGCTCCTGATCTATG20690.139731208212332No Hit
GCATAGATCAGGAGCTTAGGGGCTGTCCCTGGTTTCTGCTGATACCAATTTAAATAGTTGCTAACGCTCTGAC19850.1340582157087864No Hit
GAGTGAAGTGTGTCCCAGACCCACTGCCACTGAATCTTGACGGGACCCCA19600.13236982508273115No Hit
TCTGAGGGACCATGTAACTCTGCTGACAGTAGTAAGTTGCAAAATCTTCA19580.1322347538326467No Hit
GTCCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC19550.13203214695752008No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19060.1287229013304518No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT19020.12845275883028298No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCC18860.12737218882960763No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTC18860.12737218882960763No Hit
GTTACATGGTCCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAA18860.12737218882960763No Hit
GTCTGGGACACACTTCACTCTCACCATCAGCGGTCTGCAACCTGAAGATT18780.12683190382926995No Hit
GTACCATAGTAAGACGCTGAGGCTTTCGAACAATAATATATGGCCGTGTC18660.12602147632876343No Hit
GCCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCGTCAAGATTCAGTGGCAGTGGGT18410.12433308570270818No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC18360.12399540757749714No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18090.12217194570135746No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC17690.11947052069966907No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTCGCAACTATGCCATGAGCTGGGTCCGCCAGGCCCCAGGGAAGGGG17620.1189977713243736No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC17400.11751198757344498No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17100.1154859188221787No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16700.11278449382049031No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16150.10907003444316878No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT16000.10805700006753563No Hit
ACTTACTACTGTCAGCAGAGTTACATGGTCCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAA16000.10805700006753563No Hit
CCATGTAACTCTGCTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGA15710.10609846694131154No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15630.10555818194097387No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15320.10346457756466534No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15260.10305936381441211No Hit
GCTTAGGGGCTGTCCCTGGTTTCTGCTGATACCAATTTAAATAGTTGCTA15220.10278922131424326No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15200.10265415006415884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC3300.043.9516241
TATCTCG2600.043.59712236
TCGTATG3200.043.12112440
GTCGTAT405.0468134E-543.06089439
ATGCCGT3400.042.7948444
GTGGTAT3150.042.2848931
TATACAC3900.041.1589663
ACGAGAC3850.040.80640422
ATCTCGT2700.040.73789637
CGAGACG3900.040.28596523
GACGGAC3450.039.61662726
CACGAGA4000.039.27483721
AGACGGA3750.039.17422525
GTATGCC3800.039.12003742
GTATCAA27800.039.0673141
ACGGACT3550.038.50457427
TGTCGTA451.0188547E-438.22295838
ATGTCGT451.02376296E-438.19177637
GCCGTCT3550.038.15834846
CGGACTC3600.037.98390228
Produced by FastQC (version 0.11.5)