FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1480700
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44850.3028972783143108No Hit
GAGTTACATGGTCCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT35100.23705004389815626No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33140.22381306138988316No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31600.21341257513338285No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG30630.20686161950428852No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30160.20368744512730463No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG30140.20355237387722022No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28720.19396231512122644No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26950.18200850948875533No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCGTCAAGATTCAGTGGCAGTGGGTCTGGGACACACTTCA26480.17883433511177146No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24320.16424664010265416No Hit
CACTTCACTCTCACCATCAGCGGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAGCAGAGTTACATGGTC24240.16370635510231646No Hit
ATGTAACTCTGCTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAAGTGTGTC23240.1569527925980955No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA22620.1527655838454785No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGCCTTTTT22180.14979401634362127No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21980.14844330384277707No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21910.14797055446748159No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAGGTCAGAGCGTTAGCAACTATTTAAATTGGTATCAGCAGAAA21710.1466198419666374No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21510.1452691294657932No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21310.143918416964949No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20990.1417572769635983No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20880.141014385088134No Hit
GGTCAGAGCGTTAGCAACTATTTAAATTGGTATCAGCAGAAACCAGGGACAGCCCCTAAGCTCCTGATCTATG20040.13534139258458835No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19720.13318025258323765No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAGGTCAGAGCGTTAGCAA19650.1327075032079422No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19450.131356790707098No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18910.12770986695481865No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18740.1265617613291011No Hit
GAGTGAAGTGTGTCCCAGACCCACTGCCACTGAATCTTGACGGGACCCCA18660.12602147632876343No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18650.1259539407037212No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTC18570.12541365570338353No Hit
GTCCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC18530.12514351320321468No Hit
GCATAGATCAGGAGCTTAGGGGCTGTCCCTGGTTTCTGCTGATACCAATTTAAATAGTTGCTAACGCTCTGAC18520.1250759775781725No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18420.1244006213277504No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG18400.12426555007766597No Hit
GTCTGGGACACACTTCACTCTCACCATCAGCGGTCTGCAACCTGAAGATT18370.12406294320253934No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18230.1231174444519484No Hit
GTACCATAGTAAGACGCTGAGGCTTTCGAACAATAATATATGGCCGTGTC17990.12149658945093536No Hit
TCTGAGGGACCATGTAACTCTGCTGACAGTAGTAAGTTGCAAAATCTTCA17720.1196731275747957No Hit
GCCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCGTCAAGATTCAGTGGCAGTGGGT17430.11771459444857163No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17350.11717430944823395No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTCGCAACTATGCCATGAGCTGGGTCCGCCAGGCCCCAGGGAAGGGG16890.11406767069629228No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC16880.11400013507125008No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC16730.11298710069561693No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCC16390.11069088944418182No Hit
GTGGGGTCCCGTCAAGATTCAGTGGCAGTGGGTCTGGGACACACTTCACT16160.10913757006821098No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15800.10670628756669143No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT15630.10555818194097387No Hit
GTTACATGGTCCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAA15610.10542311069088944No Hit
GCTTAGGGGCTGTCCCTGGTTTCTGCTGATACCAATTTAAATAGTTGCTA15260.10305936381441211No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14830.10015533193759708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4600.043.765331
GTATCAA30700.038.5681
TATCAAC34950.033.3831142
ATCAACG35700.032.47533
TCAACGC35800.032.376934
CAACGCA36150.032.155595
AACGCAG36300.032.1166276
AATGGTA4550.032.0687470
ACAGGTA2000.030.709911
ATTCACT2000.030.7036842
ACGCAGA37950.030.6304247
GACAGGT2350.030.492111
CGCAGAG38550.029.8884038
AGAGTAC38650.029.5414911
TCCGGAA2402.4901965E-928.95094770
GACAGCT3000.028.43511
GCTTACC500.00816266227.2976971
ATAATAC500.00818962627.2792533
GCTTGTA1058.46132E-725.9978081
GACAGGC3050.025.7314381
Produced by FastQC (version 0.11.5)