FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1531461
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCGTCGGCTCGCAGGCTGTTCATTTGCAAATACA84110.5492141164548101No Hit
GTGTATTACTGTGCGAGAGAGAGAGCAGCTCGGGCCTTCCTCCTAGACAA51620.3370637580715408No Hit
GTATTACTGTCAGTGTTATGGTAGCTCACCTCAACGCAGTTTTGGCCAGGGGACCAAGCTGCAGATCACACGAA45070.2942941413460741No Hit
GTGTATTACTGTCAGTGTTATGGTAGCTCACCTCAACGCAGTTTTGGCCA40770.266216377694241No Hit
GTCCCTATACCATGAACTGGGTCCGCCAGGGTCCAGGGAAGGGGCTGGAG40170.2622985502079387No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37500.24486421789389348No Hit
CAGTAATACACAGCCGTGTCGTCGGCTCGCAGGCTGTTCATTTGCAAATA34770.22703810283121803No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA32340.21117090151169374No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT29970.19569548294079966No Hit
CAGTAATACACTGCGAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA29830.1947813231939958No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28830.1882516107168253No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA28720.18753334234433655No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG27830.18172189823965482No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG27510.17963239024696026No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG26680.17421272889090875No Hit
GTGTTATGGTAGCTCACCTCAACGCAGTTTTGGCCAGGGGACCAAGCTGC26230.171274358276182No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG26170.1708825755275518No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC25860.16885836465962895No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT25730.1680095020375968No Hit
GTAATACACTGCGAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA25350.165528211296272No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24790.16187157230905652No Hit
GTATTACTGTGCGAGAGAGAGAGCAGCTCGGGCCTTCCTCCTAGACAACTGGGGCCAGGGAACCCTGGTCACCGT24360.1590637959438732No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22540.14717971923542292No Hit
GTCTCCAGGCATCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAACAA21760.1420865435032299No Hit
GTCCAGGGAAGGGGCTGGAGTGGGTCTCGTCTATAAGTAGTGATGGTAGT21610.14110708663165433No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAACAACAACAACTTAGCCTGGTACC21530.14058470963348071No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21440.13999703551053538No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG21220.13856049876555784No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC21110.1378422303930691No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC21020.13725455627012376No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT20790.13575272240037456No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG20520.1339897000315385No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT20470.13366321440767998No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20400.13320613453427804No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT20380.13307554028473464No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC19590.12791706742776995No Hit
GCCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGC19400.12667642205710755No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19360.12641523355802076No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT19100.12471750831395641No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA19070.12452161693964131No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC18890.12334626869375061No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18850.1230850801946638No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC18830.12295448594512037No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18640.12171384057445798No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG18350.11982022395607855No Hit
GTCTAGGAGGAAGGCCCGAGCTGCTCTCTCTCTCGCACAGTAATACACAGCCGTGTCGTCGGCTCGCAGGCTGTT17910.11694715046612351No Hit
CCATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGT17430.1138128884770817No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC17400.11361699710276657No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT17370.11342110572845146No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16910.11041743798895302No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG16790.10963387249169258No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16790.10963387249169258No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT16610.10845852424580188No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16600.10839322712103017No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16550.10806674149717165No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC16400.10708728462559608No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTGTTGTTGTTAACACTCTGA16240.10604253062924879No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG16130.10532426225676006No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16020.1046059938842713No Hit
GGGTTCCCTGGCCCCAGTTGTCTAGGAGGAAGGCCCGAGCTGCTCTCTCTCTCGCACAGTAATACACAGCCGTGT16010.10454069675949959No Hit
ACCATAACACTGACAGTAATACACTGCGAAATCTTCAGGCTCCAGTCTGC15900.10382242838701083No Hit
TCCCTGGACCCTGGCGGACCCAGTTCATGGTATAGGGACTGAAGGTGAAT15840.1034306456383806No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT15610.10192881176863139No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15480.10107994914659922No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG15420.10068816639796901No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT15410.1006228692731973No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT15400.10055757214842559No Hit
GTCTGGGGGAGGCCTGGTCAAGCCGGGGGGGTCCCTGAGAATCTCCTGTGCAGCCTCTGGATTCACCTTCAGTCC15400.10055757214842559No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT15370.10036168077411048No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA15360.10029638364933878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)