FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1531461
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCGTCGGCTCGCAGGCTGTTCATTTGCAAATACA65040.42469249951516885No Hit
GTGTATTACTGTGCGAGAGAGAGAGCAGCTCGGGCCTTCCTCCTAGACAA48580.3172134321409425No Hit
GTATTACTGTCAGTGTTATGGTAGCTCACCTCAACGCAGTTTTGGCCAGGGGACCAAGCTGCAGATCACACGAA42390.27679451190725723No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40010.2612537962115914No Hit
GTGTATTACTGTCAGTGTTATGGTAGCTCACCTCAACGCAGTTTTGGCCA39760.2596213680922988No Hit
GTCCCTATACCATGAACTGGGTCCGCCAGGGTCCAGGGAAGGGGCTGGAG39040.25491997510873604No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35930.23461256930473579No Hit
CAGTAATACACAGCCGTGTCGTCGGCTCGCAGGCTGTTCATTTGCAAATA27380.17878352762492808No Hit
CAGTAATACACTGCGAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA27230.1778040707533525No Hit
GTGTTATGGTAGCTCACCTCAACGCAGTTTTGGCCAGGGGACCAAGCTGC27070.17675931675700524No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA26260.17147024965049715No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC25910.16918485028348745No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT24290.15860671607047125No Hit
GTAATACACTGCGAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA24050.15703958507595034No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23770.1552112655823426No Hit
GTATTACTGTGCGAGAGAGAGAGCAGCTCGGGCCTTCCTCCTAGACAACTGGGGCCAGGGAACCCTGGTCACCGT23300.15214230071807247No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG22480.14678793648679267No Hit
CTCCAGAGACAACGCCAAGAACTCGTTGTATTTGCAAATGAACAGCCTGCGAGCCGACGACACGGCTGTGTATTA21940.1432618917491206No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG21710.14176005787937138No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21580.14091119525733922No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21470.14019292688485047No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21440.13999703551053538No Hit
GTCTCCAGGCATCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAACAA20730.1353609396517443No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20340.13281435178564782No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20160.13163900353975713No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAACAACAACAACTTAGCCTGGTACC20110.1313125179158986No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19740.12889652429934553No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19580.12785177030299824No Hit
GTCCAGGGAAGGGGCTGGAGTGGGTCTCGTCTATAAGTAGTGATGGTAGT19320.12615404505893393No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA19310.12608874793416222No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC19230.12556637093598857No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT19180.12523988531213004No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18960.12380334856715255No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18370.11995081820562195No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC17580.11479234534865726No Hit
GCCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGC17150.11198456898347395No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG16840.1099603581155511No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16630.1085891184953453No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA16440.10734847312468289No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG16420.10721787887513949No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC16300.10643431337787902No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16030.10467129100904302No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT15850.10349594276315231No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT15680.10238589164203332No Hit
GTCTGGGGGAGGCCTGGTCAAGCCGGGGGGGTCCCTGAGAATCTCCTGTGCAGCCTCTGGATTCACCTTCAGTCC15590.10179821751908798No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT15460.10094935489705582No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG15460.10094935489705582No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT15360.10029638364933878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG21350.046.78377570
GGTATCA14750.044.1967161
GTATCAA36600.038.420661
TATCAAC42550.033.036212
ATCAACG43750.032.0447583
GTCACCG23750.031.24171470
CGTTGTC20700.030.31999263
CGCAGAG46950.029.9902928
GCGTTGT21150.029.60612562
AGAGTAC47600.029.50624711
GCAGAGT48350.029.192429
GGCGTTG21650.028.85845861
GTCGGAC605.795105E-428.4257533
ACGGGTA2650.028.32218616
TGGCGTT22350.028.20706660
TTGGCGT22000.028.12324359
TGTCTCT22800.028.02646366
CGAGTTC22000.027.65629652
ATTGGCG905.791333E-627.44901858
TAGTACG500.00819150827.2780274
Produced by FastQC (version 0.11.5)