FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1518114
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAAGTCCCCCGTGGACGTTCGGCCAAGGGACCCAGGTGGAAATCAAACGAACTGTGGCTGCACCAT94070.6196504346840883No Hit
CTTTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCACGTTGCTGATAATGAGACTGAATTCTGTC84330.5554918800564385No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT68190.4491757535995321No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC56000.3688787535059949No Hit
GTCTGCATCTGTAGGAGACAGAGTCAGCATCACTTGCCGGGCCAGTCAGA52730.34733886914948414No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG49860.32843383303230195No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44820.2952347452167624No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40780.26862277799954415No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG38500.2536041430353715No Hit
GAGTATCAGCAAGTGGTTGGCCTGGTATCAGCAGAGACCAGGGAAAGCCCCTAACCTCCTACTCTATGATGCCTC36310.2391783489250478No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35670.2349625917421221No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG34800.22923179682158257No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG34680.228441342349784No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTGGGTCCCTTGGCCGAACGTC33170.2184947902463188No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32860.2164527828608392No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32420.2135544497975778No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTGGGTCC31940.21039263191038352No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31690.20874585176080318No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACAT31010.20426660975394467No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT30680.20209285995649867No Hit
GTAGGAGACAGAGTCAGCATCACTTGCCGGGCCAGTCAGAGTATCAGCAAGTGGTTGGCCTGGTATCAGCAGAGA30470.20070956463085118No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30430.2004460798069183No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30280.19945801171717012No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30040.197877102773573No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28990.1909606261453356No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC28720.18918210358378884No Hit
GAGTAGGAGGTTAGGGGCTTTCCCTGGTCTCTGCTGATACCAGGCCAACC27730.18266085419145073No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC27620.18193627092563536No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT26780.17640308962304543No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26690.17581024876919651No Hit
GACTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG25340.1669176359614627No Hit
GACCAGGGAAAGCCCCTAACCTCCTACTCTATGATGCCTCCAGTTTGGAA25050.16500737098794951No Hit
GGTATCAGCAGAGACCAGGGAAAGCCCCTAACCTCCTACTCTATGATGCCTCCAGTTTGGAAAGTGGGGTCCCAT24850.16368994686828525No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACATCCAGATGACCCAGTCTCCTTCCACG24540.16164793948280565No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24510.161450325864856No Hit
GTGTGACATCCAGATGACCCAGTCTCCTTCCACGCTGTCTGCATCTGTAG23500.15479733406055146No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23160.1525577130571222No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22700.14952763758189436No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22680.14939589516992796No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22490.1481443422562469No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22000.14491665316306943No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21890.14419206989725408No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21390.14089850959809344No Hit
CAGTATAAAAGTCCCCCGTGGACGTTCGGCCAAGGGACCCAGGTGGAAATCAAACGAACTGTGGCTGCACCAT21290.1402397975382613No Hit
ACTCTATGATGCCTCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCG21130.13918585824252988No Hit
ACCTTGATGGGACCCCACTTTCCAAACTGGAGGCATCATAGAGTAGGAGGTTAGGGGCTTTCCCTGGTCTCTG21050.13865888859466416No Hit
ACTTATTACTGCCAACAGTATAAAAGTCCCCCGTGGACGTTCGGCCAAGGGACCCAGGTGGAAATCAAACGAA20720.13648513879721813No Hit
GAATTCAGTCTCATTATCAGCAACGTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAAAAGTCCC20170.1328622224681414No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCAGTCTCATTATCAGCAACGTGCAGCCTGATGATTTTG20120.13253286643822532No Hit
GTATCAGCAGAGACCAGGGAAAGCCCCTAACCTCCTACTCTATGATGCCT20080.13226938161429247No Hit
GGTTAGGGGCTTTCCCTGGTCTCTGCTGATACCAGGCCAACCACTTGCTG19660.12950279096299752No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19540.12871233649119895No Hit
GTCTCCTTCCACGCTGTCTGCATCTGTAGGAGACAGAGTCAGCATCACTT19520.12858059407923253No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA19010.12522116257408863No Hit
TCCCTGGTCTCTGCTGATACCAGGCCAACCACTTGCTGATACTCTGACTGGCCCGGCAAGTGATGCTGACT18570.12232282951082725No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18500.12186173106894477No Hit
CTGCTGATACCAGGCCAACCACTTGCTGATACTCTGACTGGCCCGGCAAG18470.12166411745099513No Hit
TCATAGAGTAGGAGGTTAGGGGCTTTCCCTGGTCTCTGCTGATACCAGGC18410.12126889021509583No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18180.11975385247748192No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17930.11810707232790159No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17790.1171848754441366No Hit
CCTTCCACGCTGTCTGCATCTGTAGGAGACAGAGTCAGCATCACTTGCCG17520.11540635288258985No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17500.11527461047062341No Hit
CATTATCAGCAACGTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAAAAGTCCCCCGTGGACGTT17480.11514286805865699No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG17190.11323260308514381No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17190.11323260308514381No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17090.11257389102531166No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16830.11086123966974812No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16700.11000491399196634No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC16690.10993904278598313No Hit
TTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCACGTTGC16660.10974142916803349No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16610.10941207313811742No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC16570.10914858831418459No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCACGTTGCTGATAATGAGACTGAATTCTGTCCCAGATCCACTGCCG16470.10848987625435244No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16460.10842400504836923No Hit
GCTGATACCAGGCCAACCACTTGCTGATACTCTGACTGGCCCGGCAAGTGATGCTGACTCTGTCTCCTACAGATG16340.10763355057657067No Hit
CTCTATGATGCCTCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCAGT16290.1073041945466546No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16010.10545980077912463No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15940.10499870233724212No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15920.10486695992527571No Hit
CTGATACTCTGACTGGCCCGGCAAGTGATGCTGACTCTGTCTCCTACAGA15880.10460347510134285No Hit
CCTCCTACTCTATGATGCCTCCAGTTTGGAAAGTGGGGTCCCATCAAGGT15810.10414237665946037No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15700.10341779339364501No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15700.10341779339364501No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15480.10196862686201431No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15350.10111230118423253No Hit
GCCAAGGGACCCAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC15260.10051946033038361No Hit
GTAATACACGGCCGTGTCAGCGGCAGTCACAGAGCTCAGCCTGAGGGAGA15220.10025597550645078No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)