FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1518114
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAAGTCCCCCGTGGACGTTCGGCCAAGGGACCCAGGTGGAAATCAAACGAACTGTGGCTGCACCAT86920.5725525224060908No Hit
CTTTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCACGTTGCTGATAATGAGACTGAATTCTGTC85020.5600369932692801No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT62720.4131442039267143No Hit
GTCTGCATCTGTAGGAGACAGAGTCAGCATCACTTGCCGGGCCAGTCAGA45460.299450502399688No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44400.2924681545654674No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42830.2821263752261029No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42380.2791621709568583No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41190.27132349744485595No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35260.2322618722968104No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34360.2263334637583212No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTGGGTCCCTTGGCCGAACGTC32490.21401554823946028No Hit
GAGTATCAGCAAGTGGTTGGCCTGGTATCAGCAGAGACCAGGGAAAGCCCCTAACCTCCTACTCTATGATGCCTC31540.207757783671055No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31480.20736255643515572No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC30090.19820645880348908No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29710.19570335297612695No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29570.19478115609236196No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTGGGTCC29330.19320024714876485No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT28990.1909606261453356No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28960.19076301252738595No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG28290.18634964172651067No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27760.18285846780940035No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27560.1815410436897361No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27020.17798399856664254No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26350.17357062776576726No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC26280.17310952932388476No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26100.17192384761618693No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACAT25610.16869615852300948No Hit
GAGTAGGAGGTTAGGGGCTTTCCCTGGTCTCTGCTGATACCAGGCCAACC25160.16573195425376486No Hit
GTAGGAGACAGAGTCAGCATCACTTGCCGGGCCAGTCAGAGTATCAGCAAGTGGTTGGCCTGGTATCAGCAGAGA24580.1619114243067385No Hit
GTGTGACATCCAGATGACCCAGTCTCCTTCCACGCTGTCTGCATCTGTAG23680.15598301576824927No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC23570.15525843250243393No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22890.15077919049557542No Hit
GGTATCAGCAGAGACCAGGGAAAGCCCCTAACCTCCTACTCTATGATGCCTCCAGTTTGGAAAGTGGGGTCCCAT22670.14933002396394474No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22660.14926415275796154No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22180.1461023348707673No Hit
GACTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG22040.1451801379870023No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT22030.14511426678101907No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21690.14287464577758982No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21460.1413596080399759No Hit
GACCAGGGAAAGCCCCTAACCTCCTACTCTATGATGCCTCCAGTTTGGAA21010.1383954037707313No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACATCCAGATGACCCAGTCTCCTTCCACG21000.13832953256474811No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20110.13246699523224212No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19560.12884407890316538No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19290.12706555634161862No Hit
CAGTATAAAAGTCCCCCGTGGACGTTCGGCCAAGGGACCCAGGTGGAAATCAAACGAACTGTGGCTGCACCAT19230.12667032910571935No Hit
ACTCTATGATGCCTCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCG19050.12548464739802148No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18780.12370612483647474No Hit
CTGCTGATACCAGGCCAACCACTTGCTGATACTCTGACTGGCCCGGCAAG18750.12350851121852507No Hit
ACTTATTACTGCCAACAGTATAAAAGTCCCCCGTGGACGTTCGGCCAAGGGACCCAGGTGGAAATCAAACGAA18700.12317915518860903No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCACGTTGCTGATAATGAGACTGAATTCTGTCCCAGATCCACTGCCG18560.12225695830484404No Hit
ACCTTGATGGGACCCCACTTTCCAAACTGGAGGCATCATAGAGTAGGAGGTTAGGGGCTTTCCCTGGTCTCTG18320.12067604936124691No Hit
GAATTCAGTCTCATTATCAGCAACGTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAAAAGTCCC18100.11922688282961622No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18050.11889752679970017No Hit
GGTTAGGGGCTTTCCCTGGTCTCTGCTGATACCAGGCCAACCACTTGCTG17940.11817294353388481No Hit
TCCCTGGTCTCTGCTGATACCAGGCCAACCACTTGCTGATACTCTGACTGGCCCGGCAAGTGATGCTGACT17650.11626267856037162No Hit
GTCTCCTTCCACGCTGTCTGCATCTGTAGGAGACAGAGTCAGCATCACTT17550.11560396650053949No Hit
GTATCAGCAGAGACCAGGGAAAGCCCCTAACCTCCTACTCTATGATGCCT17400.11461589841079128No Hit
TCATAGAGTAGGAGGTTAGGGGCTTTCCCTGGTCTCTGCTGATACCAGGC17330.11415479996890879No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17300.11395718635095915No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16920.11145408052359704No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16750.11033427002188241No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCAGTCTCATTATCAGCAACGTGCAGCCTGATGATTTTG16730.11020252760991597No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16660.10974142916803349No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16650.1096755579620503No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16410.10809464901845316No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16250.10704070972272174No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16150.10638199766288961No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15980.105262187161175No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15790.10401063424749393No Hit
CATTATCAGCAACGTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAAAAGTCCCCCGTGGACGTT15730.10361540701159465No Hit
TTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCACGTTGC15580.10262733892184644No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15530.10229798289193039No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5750.043.111311
GTATCAA42950.037.3967741
TATCAAC48650.032.7209322
ATCAACG49200.032.415593
TCAACGC49700.032.0789034
AACGCAG49900.032.01626
CAACGCA49800.032.012385
TAGTCGC703.2839198E-530.27055755
ACGCAGA53150.030.0584857
CGCAGAG53800.029.6322788
AGAGTAC55050.028.83239711
GCTTGTA1302.382876E-1028.7333011
GTGCTAG2250.028.6752531
GACAGGT3700.028.4509091
GCAGAGT56000.028.4075839
ATACGTA2050.026.47026611
GAATACG2100.025.8434289
GAGTTTA4800.025.4681531
AGTACGG46350.025.3273813
TTAACGG951.1163156E-525.22433735
Produced by FastQC (version 0.11.5)