FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004686343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences618007
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC21280.3443326693710589No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGGATGTAACTGCCTGCATATGAG19530.31601583800830735No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19050.30824893569166695No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18550.3001584124451665No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17480.28284469269765555No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTTATTATTACTGCTGCTCATAT16200.2621329531866144No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA15630.2529097566856039No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15540.2514534625012338No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG15000.24271569739501334No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG14730.23834681484190307No Hit
GTCCTGGGCTCAGTTTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG14690.23769957298218303No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGGATGTAA14590.23608146833288293No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT14200.22977086020061263No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13640.22070947416453213No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13410.21698783347114192No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC12900.20873549975971148No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG12840.20776463697013142No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGGTCCTGGG11890.19239264280178056No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11860.19190721140699055No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC11720.1896418648979704No Hit
CCCCTGAGGGCCGCTTATTGACATCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA11650.18850919164346036No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11590.18753832885388028No Hit
GTTACATCCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA11510.18624384513444023No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11290.18268401490598002No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG11250.18203677304625998No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT10850.17556435444905963No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10840.17540254398412963No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10830.1752407335191996No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10710.17329900794003952No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10700.17313719747510953No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC10680.17281357654524948No Hit
GGGCACAGGGTCCTGGGCTCAGTTTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC10640.17216633468552944No Hit
TAATAAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTGGCCAGACTTGGAGCCAGAGAAG10550.17071004050115937No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10340.16731202073762919No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTACTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC10180.16472305329874903No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTACTTATAACTATGTCTCCTGGTACCAACAACA9860.15954511842098876No Hit
CCCAATGGATGTAACTGCCTGCATATGAGCAGCAGTAATAATAAGCCTCA9630.15582347772759855No Hit
GACTTGGAGCCAGAGAAGCGATCAGGGACCCCTGAGGGCCGCTTATTGAC9550.15452899400815848No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT9340.1511309742446283No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGGATGTAAC9130.1477329544810981No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9050.14643847076165803No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTGGCCAGACTTGGAGCCAGAGAAG8990.145467607972078No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8930.14449674518249792No Hit
GTAATAATAAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG8840.14304045099812784No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC8580.13883337890994762No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC8350.13511173821655742No Hit
GGATGAGGCTTATTATTACTGCTGCTCATATGCAGGCAGTTACATCCATTGGGTGTTCGGCGGAGGGACCAAG8340.13494992775162742No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCCACACGGCCTCCCTGACCATCTCTGGGCTCCAGG8330.1347881172866974No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8230.1331700126373973No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8220.1330082021724673No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC8160.13203733938288725No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGGTCCTGGGCTCAGTTTGCCCTGACTCAGC7740.12524129985582688No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7670.1241086266013168No Hit
TCCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC7640.1236231952065268No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT7520.12168146962736669No Hit
GACCATCTCTGGGCTCCAGGCTGAGGATGAGGCTTATTATTACTGCTGCT7430.1202251754429966No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC7390.11957793358327656No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT7310.1182834498638365No Hit
GGCTTATTATTACTGCTGCTCATATGCAGGCAGTTACATCCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGT7270.11763620800411645No Hit
CAGTTACATCCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT7200.11650353474960638No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC7190.11634172428467639No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7070.11439999870551627No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7070.11439999870551627No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG6930.11213465219649615No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGGATGTAACTGCCTGCATATGAGCAGCAGTAAT6910.11181103126663614No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCTCAGTTTGCCCTGACTCAGCCT6890.11148741033677613No Hit
TCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTGGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACC6860.11100197894198609No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTA6830.11051654754719606No Hit
CAGTTTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTC6830.11051654754719606No Hit
GTCAATAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCCACACGGCCTCCCTGACCAT6790.10986930568747603No Hit
ACTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCAATAAG6780.10970749522254601No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG6620.10711852778366587No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC6600.10679490685380585No Hit
GGATGTAACTGCCTGCATATGAGCAGCAGTAATAATAAGCCTCATCCTCA6560.10614766499408583No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCCACACGG6540.10582404406422581No Hit
GAGATGGTCAGGGAGGCCGTGTGGCCAGACTTGGAGCCAGAGAAGCGATC6470.10469137080971576No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG6470.10469137080971576No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC6330.10242602430069563No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA6280.10161697197604558No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA6250.10113154058125555No Hit
TATTATTACTGCTGCTCATATGCAGGCAGTTACATCCATTGGGTGTTCGG6230.10080791965139553No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)