FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences618007
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC20950.3389929240283686No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19790.32022291009648757No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18730.30307100081390664No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGGATGTAACTGCCTGCATATGAG17940.29028797408443596No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17110.2768577054952452No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG15590.25226251482588385No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15190.2457900962286835No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTTATTATTACTGCTGCTCATAT15120.24465742297417342No Hit
GTCCTGGGCTCAGTTTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG14850.2402885404210632No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG13760.2226511997436922No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13310.21536972882184183No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT12850.20792644743506142No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12700.2054992904611113No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGGTCCTGGG12290.19886506139898094No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12100.19579066256531075No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11980.19384893698615066No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG11660.18867100210839036No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC11650.18850919164346036No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11630.18818557071360034No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGGATGTAA11370.1839784986254201No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11090.17944780560737986No Hit
GTTACATCCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA11090.17944780560737986No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTACTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC10830.1752407335191996No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC10500.16990098817650934No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC10460.1692537463167893No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10360.1676356416674892No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT10300.16666477887790915No Hit
GGGCACAGGGTCCTGGGCTCAGTTTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC10300.16666477887790915No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10140.16407581143902902No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10010.16197227539493889No Hit
CCCCTGAGGGCCGCTTATTGACATCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA9910.1603541707456388No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC9640.15598528819252858No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA9280.15016011145504823No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9220.1491892486654682No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9160.14821838587588815No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTACTTATAACTATGTCTCCTGGTACCAACAACA9140.1478947649460281No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9050.14643847076165803No Hit
TAATAAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTGGCCAGACTTGGAGCCAGAGAAG9040.14627666029672803No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8830.14287864053319785No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8680.14045148355924772No Hit
GGATGTAACTGCCTGCATATGAGCAGCAGTAATAATAAGCCTCATCCTCA8620.13948062076966766No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT8350.13511173821655742No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8340.13494992775162742No Hit
GACTTGGAGCCAGAGAAGCGATCAGGGACCCCTGAGGGCCGCTTATTGAC8340.13494992775162742No Hit
GGATGAGGCTTATTATTACTGCTGCTCATATGCAGGCAGTTACATCCATTGGGTGTTCGGCGGAGGGACCAAG7920.12815388822456705No Hit
CCCAATGGATGTAACTGCCTGCATATGAGCAGCAGTAATAATAAGCCTCA7840.12685940450512695No Hit
TCCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC7710.12475586846103685No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC7680.12427043706624682No Hit
GGCTTATTATTACTGCTGCTCATATGCAGGCAGTTACATCCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGT7650.1237850056714568No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTGGCCAGACTTGGAGCCAGAGAAG7620.12329957427666675No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGGATGTAAC7560.12232871148708671No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGGATGTAACTGCCTGCATATGAGCAGCAGTAAT7550.1221669010221567No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGGTCCTGGGCTCAGTTTGCCCTGACTCAGC7480.12103422776764663No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA7330.1186070707936965No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC7290.11795982893397648No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT7240.11715077660932643No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC7200.11650353474960638No Hit
GTAATAATAAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG7130.11537086149509632No Hit
GACCATCTCTGGGCTCCAGGCTGAGGATGAGGCTTATTATTACTGCTGCT7070.11439999870551627No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6980.1129437045211462No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG6940.11229646266142618No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTA6930.11213465219649615No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCCACACGGCCTCCCTGACCATCTCTGGGCTCCAGG6850.11084016847705608No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA6800.11003111615240604No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC6780.10970749522254601No Hit
ACTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCAATAAG6780.10970749522254601No Hit
GTCAATAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCCACACGGCCTCCCTGACCAT6650.1076039591784559No Hit
TTATTACTGCTGCTCATATGCAGGCAGTTACATCCATTGGGTGTTCGGCG6520.1055004231343658No Hit
CAGTTACATCCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT6520.1055004231343658No Hit
TATTATTACTGCTGCTCATATGCAGGCAGTTACATCCATTGGGTGTTCGG6460.10452956034478574No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC6450.10436774987985574No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCTCAGTTTGCCCTGACTCAGCCT6390.10339688709027567No Hit
TCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTGGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACC6360.10291145569548564No Hit
GCTTGGTCCCTCCGCCGAACACCCAATGGATGTAACTGCCTGCATATGAGCAGCAGTAATAATAAGCCTCATCCT6220.10064610918646552No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTA200.007769610351.0413726
ATACGCT200.00777707751.0289725
GTTGAGT200.0077995150.9918028
AGACCGG450.00979391348.13020770
TCGTACA305.2029674E-447.8342762
TTTCGTA305.34023E-447.58234460
CGTACAG350.001096749241.11315563
TTCGTAC350.001129947340.86470461
GGTATCA6200.039.5351141
AAGGCGC350.001445829838.86662723
GTGGTAT2250.037.8268051
GCGCTTT451.0856798E-437.80842626
GTATCAA14450.036.046721
CGCTCTA400.002113740235.9740163
GCCTTAT400.002571486234.5651242
GATTGAC1004.3837645E-1034.0441251
CGACCCG701.0916356E-633.9945375
TGGTATC2600.032.7188262
CTTTCGT450.00395037731.66507559
TATCAAC17300.030.0937562
Produced by FastQC (version 0.11.5)