FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1387287
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGAGAAGCCTAGGAGGCTGTCCTGGTTTGCGCTGGTACCAAGCTA65470.4719283032278109No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58360.42067719224644934No Hit
CTCATGTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAG56910.4102251372643152No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC55100.39717808932109944No Hit
CTTTTATACAATGTCAACAATAAGAACTACTTAGCTTGGTACCAGCGCAA44310.3194003836264594No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42970.30974124316021123No Hit
CTCCTAGGCTTCTCATGTACTGGGCATCTACCCGGGAATCCGGGGTCCCT42350.3052720886161263No Hit
AACCAGGACAGCCTCCTAGGCTTCTCATGTACTGGGCATCTACCCGGGAA41300.297703359146305No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39510.28480047747870485No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38370.2765829997686131No Hit
GCCTAGGAGGCTGTCCTGGTTTGCGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGACATTGTATAAAAGATT36430.2625988710338957No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35340.25474180901284305No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34510.24875890857479382No Hit
GTTTATTACTGTCAGCAATATTATGATATGATCACGTTCGGCCAAGGGAC33580.2420551767586664No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32750.23607227632061714No Hit
GTATAAAAGATTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA31990.23059395784722267No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31650.22814313116175672No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31640.2280710480239489No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31300.22562022133848295No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30890.22266481268836225No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30740.22158356562124493No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28330.20421152940955983No Hit
GTCCTGGTTTGCGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGACATTG28140.20284194979121117No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGG28000.2018327858619017No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATCTTTTATACAATGTCAA26280.1894344861589563No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATCTTTTATA25860.18640699437102778No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA25500.1838120014099462No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25420.1832353363074836No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC25190.1815774241379037No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24390.17581077311327795No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC23650.1704766209154991No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22690.1635566396859482No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTA22520.16233122634321523No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21740.1567087415942051No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTACATGAGAAGCCTAGGAGGCTGTCCTGGTTTGCGCTGGTA21720.15656457531858944No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC21630.15591582707831905No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC21300.15353708353066092No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21240.153104584703814No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21200.1528162521525827No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT20860.15036542546711676No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20830.1501491760536933No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC20560.1482029313328821No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20220.14575210464741614No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20100.14488710699372226No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19690.14193169834360156No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19410.13991337048498256No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATCTTTTATACAATGTCAACAATAAGAACTA19370.13962503793375128No Hit
ATATTATGATATGATCACGTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTT19250.1387600402800574No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18850.13587671476774452No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18790.1354442159408976No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18450.13299338925543164No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG18260.13162380963708303No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18020.1298938143296953No Hit
GGATCTCTGGTGGCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG18000.12974964805407965No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17630.12708257195519024No Hit
GCTTGGTACCAGCGCAAACCAGGACAGCCTCCTAGGCTTCTCATGTACTGGGCATCTACCCGGGAATCCGGGGTC17470.1259292417502651No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17440.12571299233684163No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA17050.12290174996233656No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG17020.12268550054891308No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA17000.12254133427329744No Hit
CTACTTAGCTTGGTACCAGCGCAAACCAGGACAGCCTCCTAGGCTTCTCATGTACTGGGCATCTACCCGGGAATC16940.12210883544645053No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATGATATGATCACGTTCGG16930.12203675230864271No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16790.1210275883793332No Hit
GTCGTGTCCCTTGGCCGAACGTGATCATATCATAATATTGCTGACAGTAA16580.11951384248536892No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT16500.11893717738290636No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16420.11836051228044378No Hit
TATCATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT16400.11821634600482814No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC16300.11749551462674991No Hit
GAACTACTTAGCTTGGTACCAGCGCAAACCAGGACAGCCTCCTAGGCTTC16270.11727926521332645No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16160.1164863506974404No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16130.11627010128401694No Hit
ATTATGATATGATCACGTTCGGCCAAGGGACACGACTGGAGATTAAACGA15980.11518885421689962No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15920.11475635539005266No Hit
TCATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC15650.11281011066924147No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA15500.11172886360212414No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15480.1115846973265085No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15470.11151261418870069No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT15440.11129636477527723No Hit
GTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCT15420.11115219849966156No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15420.11115219849966156No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATGATATGATCA15410.11108011536185373No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC15410.11108011536185373No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15190.10949428633008167No Hit
GCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTC15150.10920595377885037No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15100.10884553808981126No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG14810.10675512709338443No Hit
GAATCGGTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTACATGAGAAGCCTAGGAGGCTGTCCTGGTTTGCG14450.10416013413230282No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGGCTACGGGGACATCGTGATGA14420.10394388471887937No Hit
TTATTACTGTCAGCAATATTATGATATGATCACGTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGG14390.10372763530545591No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA14390.10372763530545591No Hit
ACTTAGCTTGGTACCAGCGCAAACCAGGACAGCCTCCTAGGCTTCTCATG14280.10293472078956986No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG14270.10286263765176204No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC14180.10221388941149163No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13970.10070014351752739No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13960.10062806037971957No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG13950.10055597724191172No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)