FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992397
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCACTTGCAGATAAA42780.43107748209637875No Hit
CTCCTGTACAGCCTCTGGATACACCTTTACCAACTTTGCCATGGCCTGGGTCCGCCGGGCTCCAGGGAAGGGG34830.35096841284284414No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT26140.2634026503506157No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21360.21523644267364772No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20950.211105031554912No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19960.2011291851950379No Hit
CTTTATTACTGTGCGAAAGGATGGGGCTTTGGTGACTACTACTTTGACTC19730.19881156432355196No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19550.19699777407630212No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19140.19286636295756637No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18250.18389817784616438No Hit
CTATGTAGACTCCGTGAAGGGCCGCTTCAACATCTCCAGAGACAATTCCAAGAACACCCTTTATCTGCAAGTGAA17280.1741238637359847No Hit
GTCTACATAGTAGGTGGTAGTAGCATGAGGACTAATTGTGGAGACCCACT16160.1628380577530968No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15330.15447446939077808No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14810.149234630898723No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14360.1447001552805984No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14300.1440955585315151No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14130.14238253440911247No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA13990.1409718086612515No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC13950.14056874416186266No Hit
TGCTACTACCACCTACTATGTAGACTCCGTGAAGGGCCGCTTCAACATCT13820.13925878453884885No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13760.1386541877897656No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13740.13845265554007116No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT13650.13754576041644626No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTACAGCCTCTGGATACACCTTTACCAACTTTGCCATGGCCTGGGTCCG13430.13532890566980754No Hit
GTCTGGGACAGACTTCACTCTCACCATCAAGAAGCTGGACCCTGAAGATT13410.1351273734201131No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13350.13452277667102983No Hit
CCCTTTATCTGCAAGTGAACAGCCTGAGAGCCGAGGACACGGCCCTTTAT13120.13220515579954392No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13110.1321043896746967No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13050.13149979292561342No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT13010.13109672842622458No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12890.12988753492805802No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12750.12847680918019705No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12740.12837604305534983No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12500.1259576560590167No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAAGAGTCAAAGTAGTAGTCAC12440.12535305930993343No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12370.12464769643600294No Hit
ATGTAGACTCCGTGAAGGGCCGCTTCAACATCTCCAGAGACAATTCCAAG12120.12212854331482259No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12030.12122164819119768No Hit
AAGTAGTAGTCACCAAAGCCCCATCCTTTCGCACAGTAATAAAGGGCCGT11810.11900479344455897No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG11780.11870249507001734No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11670.117594067696698No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAAGTGTCCAGTGTGAGGTGCAACTTTTGGAGT11530.11618334194883702No Hit
GGGTTCCCTGGCCCCAAGAGTCAAAGTAGTAGTCACCAAAGCCCCATCCTTTCGCACAGTAATAAAGGGCCGTGT11380.1146718500761288No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT11260.11346265657796226No Hit
CAGTAATAAAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCACTTGCAGATA11200.11285805982887898No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11100.11185039858040682No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG11010.1109435034567819No Hit
GGATACACCTTTACCAACTTTGCCATGGCCTGGGTCCGCCGGGCTCCAGG10960.11043967283254584No Hit
CTACTACTTTGACTCTTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTT10920.110036608333157No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGCT10800.10882741483499044No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10730.10812205196105995No Hit
GTTTATTACTGTCAGCATTATGGTAGTTCACGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA10620.10701362458774058No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCTGGACCAGTCAGACTGTTACCAC10540.10620749558896288No Hit
CAATTAGTCCTCATGCTACTACCACCTACTATGTAGACTCCGTGAAGGGCCGCTTCAACATCTCCAGAGACAATT10510.10590519721442124No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10480.10560289883987961No Hit
GGGCTGGAGTGGGTCTCCACAATTAGTCCTCATGCTACTACCACCTACTA10480.10560289883987961No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT10460.10540136659018517No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC10450.10530060046533797No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG10390.10469600371625469No Hit
TTATTACTGTGCGAAAGGATGGGGCTTTGGTGACTACTACTTTGACTCTTGGGGCCAGGGAACCCTGGTCACCGT10180.1025799150944632No Hit
CTCCAGCCCCTTCCCTGGAGCCCGGCGGACCCAGGCCATGGCAAAGTTGG10120.10197531834537993No Hit
ACATAGTAGGTGGTAGTAGCATGAGGACTAATTGTGGAGACCCACTCCAGCCCCTTCCCTGGAGCCCGGCGGACC10040.10116918934660223No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT10010.10086689097206059No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10000.10076612484721337No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9990.10066535872236615No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT9990.10066535872236615No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9970.10046382647267173No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9950.1002622942229773No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTCC150.002552186867.618024
TTCGCGT350.004329708459.16887370
AATGTCG200.00644304353.5183556
ACGGTGT200.00786404350.88789430
GGTATCA8000.048.1802671
CGTATGC2350.042.54941641
TCGTATG2350.040.99371340
ATCTCGT2100.040.7332537
CGTACTA2400.039.5614729
CTTATAC2400.039.4458351
TACTAGA2400.038.2142331
ATGCCGT2550.038.11076744
ACCGTAC2500.037.9444927
GATCTCG2200.037.25806836
GTATTAA558.491103E-636.8844151
TCTTATA1100.036.8844152
ACTAGAT2400.036.83805532
GTACTAG2600.036.534930
GACCGTA2600.036.4556326
TCTGGAG16800.036.15875270
Produced by FastQC (version 0.11.5)