FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992397
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCACTTGCAGATAAA40070.40376986226278394No Hit
CTCCTGTACAGCCTCTGGATACACCTTTACCAACTTTGCCATGGCCTGGGTCCGCCGGGCTCCAGGGAAGGGG34780.3504645822186081No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26460.2666271663457266No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT25600.2579612796088662No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19950.20102841907019067No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19080.1922617662084831No Hit
CTTTATTACTGTGCGAAAGGATGGGGCTTTGGTGACTACTACTTTGACTC17810.17946446835288699No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17450.1758368878583873No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17060.171907008989346No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16360.16485338025004106No Hit
CTATGTAGACTCCGTGAAGGGCCGCTTCAACATCTCCAGAGACAATTCCAAGAACACCCTTTATCTGCAAGTGAA15590.15709438863680564No Hit
GTCTACATAGTAGGTGGTAGTAGCATGAGGACTAATTGTGGAGACCCACT14820.1493353970235702No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14380.1449016875302928No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13960.14066951028670988No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13930.1403672119121682No Hit
GTCTGGGACAGACTTCACTCTCACCATCAAGAAGCTGGACCCTGAAGATT13920.140266445787321No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13890.13996414741277935No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT13230.1333135831728633No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC13170.13270898642378No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13050.13149979292561342No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13000.13099596230137736No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA12850.12948447042866917No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12760.12857757530504427No Hit
TGCTACTACCACCTACTATGTAGACTCCGTGAAGGGCCGCTTCAACATCT12480.12575612380932227No Hit
CCCTTTATCTGCAAGTGAACAGCCTGAGAGCCGAGGACACGGCCCTTTAT12010.12102011594150325No Hit
GGGTTCCCTGGCCCCAAGAGTCAAAGTAGTAGTCACCAAAGCCCCATCCTTTCGCACAGTAATAAAGGGCCGTGT11980.12071781756696162No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11920.12011322081787834No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11790.11880326119486455No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11780.11870249507001734No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG11630.11719100319730914No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT11410.11497414845067044No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11370.11457108395128159No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG11270.11356342270280946No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTACAGCCTCTGGATACACCTTTACCAACTTTGCCATGGCCTGGGTCCG11250.11336189045311504No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAAGTGTCCAGTGTGAGGTGCAACTTTTGGAGT11240.11326112432826782No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11210.11295882595372618No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11100.11185039858040682No Hit
ATGTAGACTCCGTGAAGGGCCGCTTCAACATCTCCAGAGACAATTCCAAG11090.1117496324555596No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11070.11154810020586518No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAAGAGTCAAAGTAGTAGTCAC10960.11043967283254584No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG10840.1092304793343793No Hit
CAGTAATAAAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCACTTGCAGATA10670.10751745521197664No Hit
AAGTAGTAGTCACCAAAGCCCCATCCTTTCGCACAGTAATAAAGGGCCGT10610.10691285846289338No Hit
GGGCTGGAGTGGGTCTCCACAATTAGTCCTCATGCTACTACCACCTACTA10430.10509906821564355No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGCT10350.10429293921686583No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10320.1039906408423242No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG10290.10368834246778254No Hit
TTATTACTGTGCGAAAGGATGGGGCTTTGGTGACTACTACTTTGACTCTTGGGGCCAGGGAACCCTGGTCACCGT10270.10348681021808812No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT10060.10137072159629665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8600.039.682731
AATCGTT853.1195668E-936.3433239
TTAATCG853.2359821E-936.20788237
GTATCAA21300.035.6928751
TAATCGT807.5428034E-834.25929338
TCACGTA808.4713975E-833.8097225
TCTGGAG17200.032.1695570
ATAGCGG801.7572929E-631.98744863
TCCGGTC951.0888471E-831.9753918
ACGTAGG851.424196E-731.8675627
AATAGCG801.8119263E-631.86225362
TATCAAC23850.031.4405352
AATGGTA4200.030.79682570
CGTTGAA802.7273454E-630.23511742
TAGCGGC852.745055E-630.20969664
ATCAACG24850.030.1646753
TCAACGC24800.029.944134
CAACGCA25250.029.5411825
TTTAAGT2452.1609594E-929.3303170
AACGCAG25550.029.1943176
Produced by FastQC (version 0.11.5)