FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1603685
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56700.35356070550014496No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50870.3172069327829343No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37960.23670483916729282No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG36420.22710195580802964No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36400.22697724303713013No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35920.22398413653554158No Hit
GTGTTAACACCTTCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC35870.2236723546082928No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA32600.20328181656622094No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGTGAAAGAGCCACCCTCTCCTGCAGGGCCAGCCAGAGTGTTAACAC30770.19187059802891468No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30090.1876303638183309No Hit
GTGCAATCCCGATCGCCTTCGGCCAAGGGACACGACTGGAGATTAAACGA29890.1863832361093357No Hit
GTCTGGGACAGACTTCACTCTCATCATCAGCAGCCTAGAGCCTGAAGATT29630.1847619700876419No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29490.18388898069134524No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29090.18139472527335482No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28940.1804593794916084No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGATGAGAGTGAAGTCTGTCCCAGACCCACTGCCA28650.17865104431356532No Hit
CTCCTGCAGGGCCAGCCAGAGTGTTAACACCTTCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT28090.1751590867283787No Hit
GTCCCAGACCCACTGCCACTGAATCTGGCTGGGATGCCAGTGGCCCTGTT27520.17160477275774233No Hit
GTTTATTACTGTCTGCAGCGTGCAATCCCGATCGCCTTCGGCCAAGGGAC26220.16349844264927338No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26190.1633113734929241No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25300.15776165518789537No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24710.15408262844635948No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24190.1508400964029719No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23790.14834584098498146No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23320.1454150908688427No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22300.13905473955296707No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCTGCAGCGTGCAATC21510.13412858510243594No Hit
GGATTGCACGCTGCAGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGATGAGAGTGAAGT21480.13394151594608666No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20870.13013777643365126No Hit
GTCTTTGTCTCCAGGTGAAAGAGCCACCCTCTCCTGCAGGGCCAGCCAGAGTGTTAACACCTTCTTAGCCTGGTA20330.12677053161936414No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19640.12246794102333063No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19400.12097138777253638No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18830.1174170738019No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18640.11623230247835455No Hit
TTCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCA18410.11479810561301003No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18250.11380040344581385No Hit
GTGTTAACACTCTGGCTGGCCCTGCAGGAGAGGGTGGCTCTTTCACCTGGAGACAAAGACAGGGTGGCTGGAGA18240.1137380470603641No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGATGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17920.11174264272597176No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAATCTGGCTGGGATGCCA17700.11037080224607701No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17170.10706591381723968No Hit
GACTTCACTCTCATCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTA16980.10588114249369422No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGATTCAGTGGCAGTGGGTCTGGGACAGACTTCACT16810.10482108394104826No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16790.10469637117014877No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16750.1044469456283497No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA16490.10282567960665592No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16490.10282567960665592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4700.043.5417561
GGTATCA17750.042.46651
TGGTATC4850.041.4905552
CGCTAAT1950.040.3984632
GTATCAA44100.037.3560641
TCGCTAA2200.037.3353531
TCGTATG2050.037.0331140
CGTATGC2000.036.2827741
TATCAAC50100.032.813182
TCAACGC50900.032.4324764
ATCAACG51250.032.2109873
ATGCCGT1950.032.02140844
CAACGCA52100.031.7499475
CCCACGA2900.031.7495119
AACGCAG52350.031.7276346
CACGAGA2800.031.66551821
ATCTCCG3100.030.79351410
ACGAGAC3000.030.6959822
ACTCGCT2900.030.64049329
ATCGTAT802.779234E-630.16414839
Produced by FastQC (version 0.11.5)