FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1603685
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54390.3391563804612502No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40600.25316692492602977No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37050.23103040809136455No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35810.22329821629559424No Hit
GTGTTAACACCTTCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC34030.21219877968553674No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31030.19349186405060845No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30780.19193295441436442No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA30370.18937634261092423No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGATGAGAGTGAAGTCTGTCCCAGACCCACTGCCA28050.17490966118657966No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27830.17353782070668491No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGTGAAAGAGCCACCCTCTCCTGCAGGGCCAGCCAGAGTGTTAACAC27760.1731013260085366No Hit
GGATTGCACGCTGCAGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGATGAGAGTGAAGT27710.1727895440812878No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27670.17254011853948875No Hit
GTCTGGGACAGACTTCACTCTCATCATCAGCAGCCTAGAGCCTGAAGATT27550.1717918419140916No Hit
GTGCAATCCCGATCGCCTTCGGCCAAGGGACACGACTGGAGATTAAACGA26890.1676763204744074No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26570.16568091614001504No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26280.16387258096197196No Hit
CTCCTGCAGGGCCAGCCAGAGTGTTAACACCTTCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT25830.16106654361673273No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25000.15589096362440255No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24910.15532975615535471No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24540.15302256989371354No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24530.15296021350826378No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24110.15034124531937382No Hit
GTTTATTACTGTCTGCAGCGTGCAATCCCGATCGCCTTCGGCCAAGGGAC23970.14946825592307716No Hit
GTCCCAGACCCACTGCCACTGAATCTGGCTGGGATGCCAGTGGCCCTGTT23910.1490941176103786No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23110.1441056067743977No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22960.1431702609926513No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21620.13481450534238332No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20420.127331739088412No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20050.12502455282677086No Hit
GTCTTTGTCTCCAGGTGAAAGAGCCACCCTCTCCTGCAGGGCCAGCCAGAGTGTTAACACCTTCTTAGCCTGGTA19660.12259265379423016No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18730.11679350994740238No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCTGCAGCGTGCAATC18290.1140498289876129No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18240.1137380470603641No Hit
GTGTTAACACTCTGGCTGGCCCTGCAGGAGAGGGTGGCTCTTTCACCTGGAGACAAAGACAGGGTGGCTGGAGA17790.11093200971512485No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17710.11043315863152676No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16970.10581878610824444No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAATCTGGCTGGGATGCCA16960.10575642972279468No Hit
TTCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCA16940.10563171695189516No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGATGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16760.10450930201379947No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16650.1038233817738521No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16470.10270096683575639No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)