FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1703626
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTATTACTGCCTGCTCTACTATGACAATACTTGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAG49660.29149590344359616No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCA47800.28057801418856015No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAAGTATTGTCATAGTAGAGCAGGCAGTAATACTCAG44840.26320330870742753No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC44770.2627924204021305No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG43660.2562769058467058No Hit
GTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAG38880.2282191044278498No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT36640.21507067865834403No Hit
GATTTATACTACAAACAACAAATACTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGC35590.2089073540788882No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG34820.2043875827206206No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC34510.20256793451144794No Hit
GTATTACTGCCTGCTCTACTATGACAATACTTGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCC34510.20256793451144794No Hit
CAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGC32970.19352839179491274No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT32450.1904760786698489No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA32010.18789335217941028No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA31130.18272789919853302No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC30690.18014517270809438No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG30310.17791463619362466No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT30070.17650587628974904No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT29650.1740405464579667No Hit
GTATAAATCAGTGACCTTGGTGCTTGTCCAGGTTTCTTCTGGAACCAGTTTGCAAAGCAACCACTGGTGACTGCT29410.17263178655409112No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTG28940.16987296507566801No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG28260.16588147868135378No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27960.16412052880150924No Hit
CCCAAGTATTGTCATAGTAGAGCAGGCAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGG27270.16007034407786686No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27200.15965945577256982No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC27060.1588376791619757No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG26940.15813329921003788No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA26890.15783980756339713No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTGTGCAGC26880.15778110923406896No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA26220.153907019498411No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG25810.15150038799595686No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGCACTGGAGCAGTCACCAGTGGTTG25360.14885896317619007No Hit
GTCCAGGAGTATTTGTTGTTTGTAGTATAAATCAGTGACCTTGGTGCTTG24420.14334132021934393No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24110.14152167201017124No Hit
CAACAAATACTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGG23880.14017161043562376No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23600.13852805721443556No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG23460.13770628060384144No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG23100.13559314074802803No Hit
GCCTGAGGACGAGGCTGAGTATTACTGCCTGCTCTACTATGACAATACTT23010.13506485578407468No Hit
CACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGC22870.13424307917348058No Hit
ATACTTGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCC22160.13007549779118188No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21780.12784496127671213No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA21740.12761016795939953No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21300.12502744146896091No Hit
GTCCAATTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC21190.12438175984635126No Hit
TCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGGTC21070.12367737989441344No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACAC21040.123501284906429No Hit
CCTGACAGTGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCG20780.12197512834389707No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC20700.12150554170927187No Hit
GTGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGG20250.1188641168895051No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20250.1188641168895051No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20200.11857062524286434No Hit
CACCTGTGCTTCCAGCACTGGAGCAGTCACCAGTGGTTGCTTTGCAAACT19870.11663358037503538No Hit
GCACTGGAGCAGTCACCAGTGGTTGCTTTGCAAACTGGTTCCAGAAGAAA19860.11657488204570722No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAAGTATTGTCATAGTAGAGCAGGCAGTAATACTCAGC19690.11557701044712866No Hit
GCCCTGACACTGTCAGGTGTGCAGCCTGAGGACGAGGCTGAGTATTACTG19590.11499002715384715No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19000.11152682572348625No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCTCAGAC19000.11152682572348625No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC18310.10747664099984386No Hit
GTATTTGTTGTTTGTAGTATAAATCAGTGACCTTGGTGCTTGTCCAGGTT18310.10747664099984386No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT18100.1062439760839527No Hit
CCTGAGAACCGGGCAGGGGTCCAGGAGTATTTGTTGTTTGTAGTATAAAT17980.10553959613201488No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17340.10178290305501325No Hit
CCTTGGTGCTTGTCCAGGTTTCTTCTGGAACCAGTTTGCAAAGCAACCAC17300.10154810973770063No Hit
CTCTCACCTGTGCTTCCAGCACTGGAGCAGTCACCAGTGGTTGCTTTGCA17080.10025674649248133No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATT252.5614325E-455.2265740
TAAGCGT250.007424950651.63390469
AGGGGGG2850.050.30806770
TCGTATG3200.042.06711240
AATCTCG2950.041.9377236
TCTCGTA2900.041.5469838
ATCTCGT2950.040.81017337
ACGAGAC4150.040.30351622
CGTATGC3350.040.23965541
GTGGTAT5000.039.5949861
TATACAC4500.038.6847573
GTATCAA36950.038.613931
CACGAGA4350.037.66356321
GCCGTCT3550.037.2191446
GCAATCT3500.036.27470434
CGAGACA4650.035.9708623
AGGCAAT4400.035.82394832
TTATACA4800.035.5558432
CTTATAC4800.034.8447271
GAGGCAA4650.034.6134231
Produced by FastQC (version 0.11.5)