FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1703626
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTATTACTGCCTGCTCTACTATGACAATACTTGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAG48750.28615435547473445No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48250.28321943900832697No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG44350.2603270905703482No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC43810.25715738078662803No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCA42550.24976139129128105No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAAGTATTGTCATAGTAGAGCAGGCAGTAATACTCAG39610.2325040824688048No Hit
GATTTATACTACAAACAACAAATACTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGC36780.21589245526893813No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA36080.2117835722159676No Hit
GTATTACTGCCTGCTCTACTATGACAATACTTGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCC35140.20626592925912143No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33170.19470235838147573No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT32760.19229572687902158No Hit
GTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAG32480.19065217365783335No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC32090.18836293881403546No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG29880.1753906080325142No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG29410.17263178655409112No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT29260.17175131161416884No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC28410.16676195362127605No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTG28380.16658585863329156No Hit
GTATAAATCAGTGACCTTGGTGCTTGTCCAGGTTTCTTCTGGAACCAGTTTGCAAAGCAACCACTGGTGACTGCT28300.16611627199866638No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA28230.16570538369336932No Hit
CAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGC27880.16365094216688406No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG27590.16194869061636766No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTGTGCAGC27550.16171389729905508No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26450.15525708107295852No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26240.15402441615706733No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACAC25390.14903505816417453No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25060.14709801329634556No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24650.14469138179389138No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG24620.14451528680590695No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC24040.1411107837048742No Hit
CCCAAGTATTGTCATAGTAGAGCAGGCAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGG23730.13929113549570155No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23420.13747148728652886No Hit
GTCCAGGAGTATTTGTTGTTTGTAGTATAAATCAGTGACCTTGGTGCTTG23340.13700190065190365No Hit
CAACAAATACTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGG23030.13518225244273097No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23010.13506485578407468No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22940.13465396747877761No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGCACTGGAGCAGTCACCAGTGGTTG22430.13166035268304194No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG22360.13124946437774487No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21440.12584921807955501No Hit
GCCTGAGGACGAGGCTGAGTATTACTGCCTGCTCTACTATGACAATACTT21350.12532093311560166No Hit
CACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGC20880.12256211163717858No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA20830.12226861999053783No Hit
ATACTTGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCC20820.12220992166120968No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20620.12103595507464666No Hit
GTCCAATTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC20220.11868802190152063No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20110.11804234027891099No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG19980.11727926199764502No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18720.10988327250229804No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18670.10958978085565728No Hit
GCACTGGAGCAGTCACCAGTGGTTGCTTTGCAAACTGGTTCCAGAAGAAA18650.10947238419700099No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18450.10829841761043797No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18280.10730054601185941No Hit
TCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGGTC17960.10542219947335858No Hit
GTGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGG17940.1053048028147023No Hit
GCCCTGACACTGTCAGGTGTGCAGCCTGAGGACGAGGCTGAGTATTACTG17710.10395474124015483No Hit
CCTGACAGTGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCG17390.10207639470165401No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17340.10178290305501325No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17310.10160680806702879No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCTCAGAC17290.1014894114083725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5300.041.21211
GTATCAA40500.037.162441
TATCAAC45950.032.817412
ATCAACG48100.031.3367083
TCAACGC48200.031.1917824
CAACGCA49300.030.4940325
AACGCAG50000.030.2034726
GATTGAC1700.030.1136231
ACGCAGA51850.029.1266737
CGCAGAG51850.029.1266738
AGAGTAC52900.028.73765611
ATAAACG605.8045174E-428.4180933
CAGAGTA53500.028.28960410
CTCGTAA500.00815524427.303021
CTCGCTA907.5659373E-626.52121723
GGTATCA27850.026.4697131
TAAACGC659.282055E-426.2243864
CGTGTCA1908.655406E-626.06287870
AGGATGG11500.025.82328669
TCGCTAT951.1481054E-525.1334824
Produced by FastQC (version 0.11.5)