FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462133
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42130.9116423194188686No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC11250.2434364133268994No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC10850.23478089640860964No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9730.21054544903739833No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG9110.1971293978140492No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG8560.18522806205140077No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT8240.178303648516769No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7920.17137923498213717No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT7240.1566648562210446No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG7200.15579930452921562No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG6810.1473601755338831No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT6790.1469273996879686No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA6630.1434651929206527No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT5870.12701971077590218No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTAGTCCCTCCGCCGACGAACACCCAACTGTAGGCGCCTGCATATGAG5760.1246394436233725No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG5730.12399027985450077No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC5630.12182640062492832No Hit
GACCTAGGACGGTCACCTTAGTCCCTCCGCCGACGAACACCCAACTGTAG5580.1207444610101421No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG5310.11490198709029652No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA5110.11057422863115164No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC5060.10949228901636543No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT5020.10862673732453644No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC4970.10754479770975023No Hit
CCTTAGTCCCTCCGCCGACGAACACCCAACTGTAGGCGCCTGCATATGAGCAGCAATAATAATCAGCCTCATCCT4790.10364981509651983No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC4690.1014859358669474No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG4690.1014859358669474No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCG200.00596760954.5603356
TTCGAAG400.006458239653.4724170
GTGGTAT2450.050.3022961
TCGCGAA200.008463765549.94618618
TGGTATC2700.044.4110342
GTATCAA19950.038.9013941
GGTATCA7650.037.0091971
TCCGAGA1301.848876E-532.90609770
TATCAAC24000.032.1979982
AACGCAG24750.031.1918936
ATCAACG25500.030.2908763
TCAACGC25700.030.042144
ACGCAGA26050.029.6352947
CGCAGAG26150.029.5219658
CAACGCA26050.029.5075555
AGAGTAC26650.028.71525211
CAGAGTA27000.028.58947810
GCAGAGT27100.028.483989
GATCCTA805.881079E-428.34642269
TCGGAAG500.006826239628.31184448
Produced by FastQC (version 0.11.5)