FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences695636
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG29190.4196160060721411No Hit
GAGTACTGCTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG25150.36153965579699726No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19830.2850628777119068No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC19270.2770126905450552No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19050.2738501170152206No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACAAGGAGTACTGCTATCCCACAC16820.24179312169007927No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16520.2374805214221231No Hit
GGCCAGGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGCCCGGCCC14680.21102990644532488No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACAAGGAGTA14150.20341097930526883No Hit
CCCCAGGACAGACGGCCACGATTACCTGTGGGGGAAACAACATTGGAAGT13910.19996089909090387No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13370.19219821860858266No Hit
CCCCTGTGCTGGTCGTCTATGATGATAGCGCCCGGCCCTCAGGGATCCCT13350.19191071192405224No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA12860.18486679815305707No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT12790.1838605247572006No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12710.18271049801907893No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG12650.18184797796548768No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12640.1817042246232225No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12460.17911666446244875No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12340.17739162435526626No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12300.1768166109862054No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12230.17581033759034898No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT12100.17394154414090127No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11970.17207275069145359No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGCCCGGCCCTCAGG11910.17121023063786234No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCACGATTAC11890.1709227239533319No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC11860.1704914639265363No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG11600.16675387702764088No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11560.16617886365858006No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11530.16574760363178442No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG11170.16057248331023696No Hit
CGATTACCTGTGGGGGAAACAACATTGGAAGTGACAATGTGCACTGGTACCAGCAGAGGCCAGGCCAGGCCC10920.15697864975360679No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10770.15482234961962865No Hit
CTATTACTGTCAGGTGTGGGATAGCAGTACTCCTTGTGTGGTATTCGGCG10770.15482234961962865No Hit
GCACAGGGGCCTGGCCTGGCCTCTGCTGGTACCAGTGCACATTGTCACTT10700.1538160762237722No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG10680.1535285695392418No Hit
GCAGTACTCCTTGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA10630.15280980282791576No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTC10570.1519472827743245No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA10300.14806594253316388No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGC10210.14677216245277702No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGGCGCTATCATCATAGA10180.1463409024259814No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC10170.1461971490837162No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA10130.14562213571465535No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10090.14504712234559455No Hit
GGATAGCAGTACTCCTTGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCC10060.14461586231879892No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGGTGTGGGATAGCAGTA10050.1444721089765337No Hit
CTGCTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGAC9850.14159704213122956No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9710.13958449533951664No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9610.13814696191686457No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG9490.13642192180968207No Hit
GGTACCAGCAGAGGCCAGGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGCCCGGCCCTCAGGGATCC9450.13584690844062125No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG9360.13455312836023436No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGT9250.13297184159531708No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG9110.1309592948036042No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCACGATTACCTGTGGGGGAAACAACATTGGAAGTGACAATGTG8920.12822798130056526No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGC8580.12334036766354817No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG8530.12262160095222213No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT8510.12233409426769173No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC8430.12118406752957007No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGC8320.11960278076465276No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC8150.11715897394614425No Hit
GGACAGACGGCCACGATTACCTGTGGGGGAAACAACATTGGAAGTGACAA7930.11399640041630968No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC7880.11327763370498364No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC7840.1127026203359228No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG7820.1124151136513924No Hit
GTGTCAGTGGCCCCAGGACAGACGGCCACGATTACCTGTGGGGGAAACAA7750.11140884025553593No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7660.11011506017514908No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7600.10925254012155783No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC7570.1088212800947622No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC7420.10666497996078408No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7390.10623371993398846No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAC7270.10450867982680596No Hit
GCTTGGTCCCTCCGCCGAATACCACACAAGGAGTACTGCTATCCCACACCTGACAGTAATAGTCGGCCTCATCCC7020.10091484627017579No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT7000.10062733958564538No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC6980.10033983290111495No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT6960.10005232621658454No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATAT554.3318057E-449.6394170
GGTATCA6400.046.0528341
ATCTCCG2050.041.7799510
GTATCAA17600.041.671491
AGTATCG503.917583E-641.28060534
GACCGAT1750.041.13512826
AGACCGA1900.039.6803725
ACGAGAC2100.039.16222822
TATACAC2500.038.381743
CCGATCA1800.038.11823728
ACCGATC1800.038.10452327
CACGAGA2200.037.3794421
CTTATAC2300.035.761961
CGATCAG2050.035.16593629
CCCACGA2350.034.9985519
TTATACA2450.034.968792
TATCAAC21300.034.7520832
ATCAACG21800.033.7978133
TCAACGC21800.033.6406144
ACACATC2550.033.5974666
Produced by FastQC (version 0.11.5)