FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences695636
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG26930.3871277507202042No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23130.33250148065942536No Hit
GAGTACTGCTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG23030.33106394723677324No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19710.2833378376047243No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17830.2563122092588653No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC15520.2231051871956023No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT14550.20916111299587714No Hit
CCCCTGTGCTGGTCGTCTATGATGATAGCGCCCGGCCCTCAGGGATCCCT14540.20901735965361193No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13900.19981714574863865No Hit
GGCCAGGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGCCCGGCCC13760.19780459895692576No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT13520.19435451874256077No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACAAGGAGTACTGCTATCCCACAC13350.19191071192405224No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA13240.19032942515913495No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG12500.1796916778315096No Hit
CCCCAGGACAGACGGCCACGATTACCTGTGGGGGAAACAACATTGGAAGT12470.17926041780471397No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12270.17638535095940983No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11910.17121023063786234No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11790.16948519053067984No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACAAGGAGTA11720.16847891713482338No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGGCGCTATCATCATAGA11570.16632261700084527No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11500.1653163436049888No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCACGATTAC11430.16431007020913235No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG11400.16387881018233674No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC11290.16229752341741946No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11060.15899119654531968No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC10860.15611612970001554No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGCCCGGCCCTCAGG10820.1555411163309547No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA10480.15065350269393762No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC10450.15022224266714201No Hit
CTGCTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGAC10330.14849720255995952No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10160.146053395741451No Hit
GCACAGGGGCCTGGCCTGGCCTCTGCTGGTACCAGTGCACATTGTCACTT10140.14576588905692056No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10120.14547838237239016No Hit
CTATTACTGTCAGGTGTGGGATAGCAGTACTCCTTGTGTGGTATTCGGCG9980.14346583558067727No Hit
GCAGTACTCCTTGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA9960.14317832889614684No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG9830.14130953544669914No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG9770.1404470153931079No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA9730.13987200202404707No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9670.13900948197045582No Hit
GGATAGCAGTACTCCTTGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCC9640.13857822194366018No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGC9620.13829071525912978No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9320.13397811499117354No Hit
CGATTACCTGTGGGGGAAACAACATTGGAAGTGACAATGTGCACTGGTACCAGCAGAGGCCAGGCCAGGCCC9300.13369060830664312No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9280.13340310162211272No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9240.13282808825305187No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTC8820.12679044787791316No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGC8670.12463414774393504No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG8620.123915381032609No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG8590.12348412100581338No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC8580.12334036766354817No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG8530.12262160095222213No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8360.1201777941337136No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8340.1198902874491832No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGGTGTGGGATAGCAGTA8280.11902776739559195No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCACGATTACCTGTGGGGGAAACAACATTGGAAGTGACAATGTG8180.11759023397293986No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC7920.11385264707404447No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGT7700.1106900735442099No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG7680.11040256685967949No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG7520.10810251338343616No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC7510.10795876004117096No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT7480.10752750001437533No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7350.10565870656492762No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC7230.10393366645774516No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAC7170.10307114640415391No Hit
GGTACCAGCAGAGGCCAGGCCAGGCCCCTGTGCTGGTCGTCTATGATGAT7150.10278363971962347No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC7130.10249613303509307No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC7130.10249613303509307No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG7080.10177736632376704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7600.043.6634561
GTATCAA19650.040.7392731
TATCAAC23100.034.4918442
TGGTATC2600.034.1957782
ATCAACG24650.032.177323
TCAACGC24850.031.906874
CAACGCA25350.031.1427275
AACGCAG25700.030.8515836
CCGCTAT950.00535796629.74876670
AGAGTAC27000.029.74587211
ACGCAGA26900.029.4753067
CGCAGAG27400.029.1868958
CAGAGTA27750.029.06508810
GCAGAGT27700.028.870799
GCAGTCT605.7275494E-428.4800918
GTATGTA500.00805736327.3684331
GCTTAGG500.00805736327.3684331
AGGTATC907.3924894E-626.5967162
GTATTAG1759.094947E-1225.4135461
TACGGGG9800.024.0662315
Produced by FastQC (version 0.11.5)