FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923498
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19420.21028740722773628No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18230.1974016186283024No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16600.17975133676521227No Hit
GGTCTATGCTGCGTCCACCTTGGAAAGTGGGGTTCCATCAAGATTCAGCGGCAGTGGGTCTGGGACAGAATTCA16120.17455370775031456No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15310.16578270878767468No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT14640.15852768495437997No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14040.15203064868575786No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12520.13557149013858177No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG12400.13427208288485734No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11810.1278833305540456No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11810.1278833305540456No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC11690.12658392330032117No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT11480.12430996060630344No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT11460.12409339273068269No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11250.12181943003666494No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10700.11586381345709466No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10630.11510582589242208No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10330.11185730775811101No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG10160.11001648081533474No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10140.109799912939714No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGACTTACAATTTC10130.10969162900190361No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGGC200.00809025450.5238415
GTGGTAT4000.038.7538261
TATCTCG3050.038.06720736
ACCGATC5750.037.5735727
TCCTATA451.1503082E-437.4412882
TCAGTAT5950.036.50473432
ATCTCGT3200.036.36065337
GACCGAT6000.035.9865226
TCGTATG2600.035.79797740
AGACCGA5950.035.7083625
GAGACCG5950.035.70061524
ACGAGAC6050.035.6504422
TATACAC6350.035.5544783
CCGATCA6300.035.38804628
CGATCAG6150.035.19087229
AGTATCG1850.034.96014434
CGTATGC2500.034.57367341
CGTTTGC501.8379156E-434.5736741
GCCGTCT2950.034.37359646
CCCACGA6300.034.22276319
Produced by FastQC (version 0.11.5)