FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923498
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63600.6886858444739458No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15670.16968093054884797No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15480.16762353573045097No Hit
GGTCTATGCTGCGTCCACCTTGGAAAGTGGGGTTCCATCAAGATTCAGCGGCAGTGGGTCTGGGACAGAATTCA14990.1623176227777429No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13900.15051467355641268No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13530.14650816785742904No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12210.13221468806646036No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT11170.120953158534182No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11070.1198703191560783No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10800.11694665283519834No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT10290.11142417200686953No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9660.1046022839248163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4300.045.3781851
GACCGGA1503.6379788E-837.210470
GTATCAA47300.032.2199331
TCGTGAT900.004375700431.00866770
AACGCAG49000.030.8660096
GGTATCA22250.030.845211
ATCAACG48400.030.769223
TCAACGC48850.030.5496314
CAACGCA49900.030.0398925
ACGCAGA52100.029.0294517
CGCAGAG52150.029.001628
AGAGTAC54350.027.94833411
GCAGAGT54400.027.8638869
CAGAGTA54800.027.7816610
CTGTACG550.00774303727.5931367
TATCAAC55250.027.3313062
CCAAACG500.0085293827.05178829
GCAACGT757.169575E-526.99296627
GGTACTA1107.291783E-726.4422760
GCTACTA650.001007018825.8591823
Produced by FastQC (version 0.11.5)