FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1385127
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATCGTACGTCACCGTGGACGTTCGGCCAAGGGA58560.42277711718853217No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43990.31758820671317506No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGACTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA39210.28307873574047726No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35820.2586044456573296No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGCCC33430.2413497101709807No Hit
GTCTCTGGTGGCTCCATCAGTAGACACTACTGGAGCTGGATCCGGCAGCC29500.2129768606055618No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28390.20496315500311524No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28230.20380802626762742No Hit
GAGTGTTACTAACTACTTAGCCTGGTACCGGCACAAACCTGGCCAGACTCCCAGGCTCCTCATCTATGGTGCATC28150.20323046189988356No Hit
GTGTATTACTGTCAGCAGTATCGTACGTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGGAATCAAACGAA27330.19731042713050864No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTAGACACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGA24280.17529078561027256No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGACTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA23750.17146442167396925No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA23560.1700927063005775No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22930.1655443869045943No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22910.16539999581265832No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22720.1640282804392666No Hit
CTCCTGCAGGGCCGATCAGAGTGTTACTAACTACTTAGCCTGGTACCGGCACAAACCTGGCCAGACTCCCAGGCT22270.16077948087070718No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC22170.1600575254110273No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21720.15680872584246788No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21600.15594237929085203No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGTCTGGAGCCTGAAGATT21560.1556535971069801No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21120.15247699308438867No Hit
GTATCGTACGTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGGAATCAAACGAACTGTGGCTGCACCAT21100.1523326019924527No Hit
GTACGATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGACTGCTGATGGTGAGAGTGAAGTCTGTC20770.1499501489755091No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20730.14966136679163716No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20370.14706232713678963No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19970.14417450529807013No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT19910.14374133202226222No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19820.14309157210855034No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19610.1415754656432226No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC19440.14034814136176685No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCGATCAGAGTGTTACTAACTACTTAGCCTGGTA19420.14020375026983087No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCGATCAGAGTGTTACTAA19290.13926520817224702No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19120.13803788389079125No Hit
GGACTGGAGTGGGTCGGGTATATCTCTGACAGTGGGAGCACCAACTACAA18980.13702714624723944No Hit
GAAATACACGGCCGTGTCTGCGGCGGTCACAGAACTCAACTTCAGGGAGA18950.13681055960933547No Hit
CCATAGATGAGGAGCCTGGGAGTCTGGCCAGGTTTGTGCCGGTACCAGGC18940.13673836406336748No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18920.1365939729714315No Hit
CTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGGTCGGGTATATCTCTGACAGTGGGAGCAC18580.13413932440851994No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17600.12706416090365721No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17340.12518707670848953No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17020.12287681923751395No Hit
CTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCA16850.12164949495605819No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGC16680.1204221706746024No Hit
GTGCAGCCACAGTTCGTTTGATTCCCACCTTGGTCCCTTGGCCGAACGTC16600.1198446063068585No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTCCCACCTTGGTCC16390.11832849984153078No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT16090.11616263346249116No Hit
CTACTTAGCCTGGTACCGGCACAAACCTGGCCAGACTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCA15740.11363578935361161No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15630.11284163834796375No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15500.11190309625037993No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA15490.11183090070441194No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15460.11161431406650799No Hit
GACTTCACTCTCACCATCAGCAGTCTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATCGTACGTCA15250.11009820760118025No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14840.10713819021649278No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14680.105983061481005No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14670.10591086593503701No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGTACCCTAGAG14610.10547769265922909No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14420.10410597728583731No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14420.10410597728583731No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14140.10208450199873369No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14000.10107376435518187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3900.040.1214521
GGTATCA12050.039.8029671
TCGTATG3800.038.99014340
ATGCCGT3750.038.8040544
GTAATCT3200.038.5155434
GTATCAA30900.038.4193761
ACGCGTA3850.038.0523627
TGGTATC4300.037.969992
CGTATGC3750.037.73720641
ATCTCGT3200.037.54936637
GACGCGT4000.036.60951226
CGTAGTA4150.035.36681430
CGCGTAG4150.035.31818828
AATCTCG3400.035.30070536
ACGAGAC4300.034.8171422
AGACGCG4250.034.4435625
GCGTAGT4300.034.11948829
TAGTAAT4350.033.78972232
CCGTCTT3950.033.48470347
GAGACGC4400.033.2573324
Produced by FastQC (version 0.11.5)