FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1385127
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATCGTACGTCACCGTGGACGTTCGGCCAAGGGA49510.3574401480875039No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41750.30141640441634593No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38410.27730309206303827No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGACTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA36030.26012055212265733No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGCCC29820.2152871180765374No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27440.19810457813615648No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27080.19550553848130892No Hit
GTCTCTGGTGGCTCCATCAGTAGACACTACTGGAGCTGGATCCGGCAGCC27040.19521675629743698No Hit
GTGTATTACTGTCAGCAGTATCGTACGTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGGAATCAAACGAA26380.19045185026354983No Hit
GAGTGTTACTAACTACTTAGCCTGGTACCGGCACAAACCTGGCCAGACTCCCAGGCTCCTCATCTATGGTGCATC26320.1900186769877419No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25240.1822215580231993No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23880.17240296377155306No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23540.1699483152086415No Hit
CTCCTGCAGGGCCGATCAGAGTGTTACTAACTACTTAGCCTGGTACCGGCACAAACCTGGCCAGACTCCCAGGCT21900.15810824566989165No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTAGACACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGA21690.1565921392045639No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGACTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA21270.15355992627390846No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGTCTGGAGCCTGAAGATT21020.1517550376247088No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC20660.14915599796986126No Hit
GTATCGTACGTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGGAATCAAACGAACTGTGGCTGCACCAT20240.1461237850392058No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA20230.14605158949323782No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCGATCAGAGTGTTACTAACTACTTAGCCTGGTA19720.14236961664887046No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19520.14092570572951071No Hit
GTACGATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGACTGCTGATGGTGAGAGTGAAGTCTGTC19460.1404925324537028No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18540.133850542224648No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18450.1332007823109361No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC18320.13226224021335228No Hit
GGACTGGAGTGGGTCGGGTATATCTCTGACAGTGGGAGCACCAACTACAA18310.1321900446673843No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCGATCAGAGTGTTACTAA18190.13132369811576844No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17880.12908563619076086No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17650.12742513863349714No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17620.1272085519955932No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT17580.12691976981172123No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17430.12583683662220144No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17170.12395975242703378No Hit
GAAATACACGGCCGTGTCTGCGGCGGTCACAGAACTCAACTTCAGGGAGA17090.12338218805928987No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16930.12222705932380207No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16720.12071095285847436No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16630.12006119294476246No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16570.11962801966895453No Hit
CTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGGTCGGGTATATCTCTGACAGTGGGAGCAC16460.1188338686633067No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16220.11710117556007499No Hit
GTGCAGCCACAGTTCGTTTGATTCCCACCTTGGTCCCTTGGCCGAACGTC16110.11630702455442714No Hit
CCATAGATGAGGAGCCTGGGAGTCTGGCCAGGTTTGTGCCGGTACCAGGC16010.11558506909474728No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15670.1131304205318357No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTCCCACCTTGGTCC15110.10908746995762844No Hit
GACTTCACTCTCACCATCAGCAGTCTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATCGTACGTCA15090.10894307886569246No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGC15080.10887088331972448No Hit
CTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCA14970.10807673231407662No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14940.10786014567617265No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT14710.10619964811890896No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA14520.1048279327455172No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14300.10323963073422149No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAGTACCCTAGAGCCAGCCACTGTTCACGATGTCTCGCACAGAAATACACGG14030.10129035099308582No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13940.10064059107937394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4400.043.2574581
TGGTATC4500.042.2839552
GGTATCA13150.039.803441
GTATCAA31900.037.9301831
GTATAAA701.0945787E-633.9881
TATCAAC35500.033.786752
GTTATTA900.003253537532.94550370
ATCAACG36600.032.765383
TCAACGC36900.032.4825674
AACGCAG38050.031.9458686
CAACGCA37850.031.8455525
AACTGTG26350.031.50765470
ACGCAGA39700.030.5326167
AGAGTAC39700.030.44269611
CGCAGAG39700.030.276048
GCAGAGT40600.029.854719
AAGCGAC500.00754969827.73777645
AATGGTA5100.027.61608570
CTCGCTA500.008312019527.19629921
TATAAGA1307.4214768E-926.1370582
Produced by FastQC (version 0.11.5)