FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1590277
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC53970.3393748384715367No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52390.3294394624332742No Hit
CTACTATTGTCTGCAGACTTATACTCCACCTAGGACGTTCGGCCAAGGGA45850.2883145514900863No Hit
GACTTATACTCCACCTAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT42970.27020449896464577No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39800.2502708647612963No Hit
GATCTATGGTGCATCCAATTTGCAAAGTGGGGTCCCATCAACGTTCAGTGGCCGTGGATCTGGGACAGAATTCA39320.24725252267372289No Hit
GTATAAGTCTGCAGACAATAGTAGGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAATTCTGTC39000.24524029461534058No Hit
CAATAGTAGGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACGGCCA38260.24058701723033157No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37850.23800885003052927No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG37070.23310404413822244No Hit
CTTATACTCCACCTAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA37030.23285251563092468No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35910.2258097174265867No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35440.22285425746583773No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35420.22272849321218882No Hit
CCATAGATCAGGAGCTTAGGGGCCTTCCCTGGTTTTTGCTGATACCAATT34410.21637739840291975No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG33520.21078088911554405No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30600.1924193080828057No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30290.19046996215124787No Hit
ACCTAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGG30070.18908655536111005No Hit
GAATTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACCTACTATTGTCTGCAGACTTATACTCCA29540.1857538026394144No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28370.17839659380095418No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28170.17713895126446524No Hit
GAGTATAAGTCTGCAGACAATAGTAGGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAATT27750.17449790193783848No Hit
GTAGGAGACAGAGTCACCCTCACTTGCCGGGCAAGTCAGACCGTTAGCATCTATTTAAATTGGTATCAGCAAAAA27560.17330314152817403No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26710.16795816074809608No Hit
GTCCTAGGTGGAGTATAAGTCTGCAGACAATAGTAGGTTGCAAAATCTTC26670.1677066322407983No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26010.16355641187038483No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG25190.15840007747078025No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCCTCACTTGCCGGGCAAGTCAGACCGTTAGCAT25150.15814854896348246No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23670.14884199419346442No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23540.14802452654474663No Hit
GAGTGAATTCTGTCCCAGATCCACGGCCACTGAACGTTGATGGGACCCCA22960.14437736318892871No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT22850.14368565979385983No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT22740.1429939563987909No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT22690.14267954576466865No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC21700.1364542152090485No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21530.13538521905303288No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21400.1345677514043151No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21120.1328070518532306No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20810.13085770592167276No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC20500.12890835999011493No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20130.12658172129761042No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20110.1264559570439615No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGGTCACGTTCAGGGAGAACTGGTTCTTGGACTTGTCTACTGA19890.1250725502538237No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC19310.12142538689800583No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18920.11897298395185242No Hit
ATAGTAGGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACGGCCA18780.11809263417631016No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18680.11746381290806569No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18210.11450835294731672No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC18180.11431970656684338No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18080.11369088529859891No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17940.11281053552305667No Hit
ACCTACTATTGTCTGCAGACTTATACTCCACCTAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA17460.10979219343548326No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17380.10928913642088767No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16780.1055162088114209No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC16780.1055162088114209No Hit
GAGTCACCCTCACTTGCCGGGCAAGTCAGACCGTTAGCATCTATTTAAATTGGTATCAGCAAAAACCAGGGAAGG16710.10507603392364978No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16690.10495026967000087No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCCTCACTTGCCGGGCAAGTCAGA16460.10350398075303863No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16400.10312668799209194No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT16390.10306380586526749No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16180.10174328120195412No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16060.10098869568006077No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT16040.10086293142641187No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT16040.10086293142641187No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT252.6173377E-454.98546239
GTCGTAT306.4344483E-445.8212239
CGTATGC4750.040.64938441
TCGTATG4500.040.5336940
TATCGTA350.001384408139.21518738
ATGCCGT4900.038.9159344
GTATCAA40650.038.772951
GCAATCG451.0657326E-437.93225534
TATACAC6000.036.077153
ACGAGAC5850.035.8616722
GTATGCC5500.035.8066542
CCGTCTT4600.035.6229247
CACGAGA5900.035.5510521
TCTCCGA5950.035.23789211
GCCGTCT4550.035.19563746
GACCGGA1409.207961E-734.74447670
GCGTATG501.8887784E-434.41539840
CGAGACA6100.034.39409623
ATCTCCG6250.034.08754710
ATCTCGT4400.033.48110237
Produced by FastQC (version 0.11.5)