FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1590277
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58840.3699984342350421No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51770.3255407705701585No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43630.2743547193350592No Hit
GACTTATACTCCACCTAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT41020.2579424842338788No Hit
CTACTATTGTCTGCAGACTTATACTCCACCTAGGACGTTCGGCCAAGGGA40970.25762807359975654No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40950.2575023093461076No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37790.2376315572695826No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG37210.23398439391376474No Hit
GTATAAGTCTGCAGACAATAGTAGGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAATTCTGTC35340.22222543619759327No Hit
GATCTATGGTGCATCCAATTTGCAAAGTGGGGTCCCATCAACGTTCAGTGGCCGTGGATCTGGGACAGAATTCA34380.21618875202244642No Hit
CAATAGTAGGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACGGCCA33930.21335905631534635No Hit
CTTATACTCCACCTAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA33400.21002630359365065No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32610.2050586155745194No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31500.19807869949700585No Hit
CCATAGATCAGGAGCTTAGGGGCCTTCCCTGGTTTTTGCTGATACCAATT30490.1917276046877368No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30130.18946384812205672No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29760.18713720942955223No Hit
GAATTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACCTACTATTGTCTGCAGACTTATACTCCA27890.17537825171338076No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27640.1738061985427696No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27280.1715424419770895No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27230.1712280313429673No Hit
ACCTAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGG26930.1693415675382339No Hit
GTCCTAGGTGGAGTATAAGTCTGCAGACAATAGTAGGTTGCAAAATCTTC26620.16739222160667605No Hit
GAGTATAAGTCTGCAGACAATAGTAGGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAATT26380.16588305056288935No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25670.16141841955835368No Hit
GTAGGAGACAGAGTCACCCTCACTTGCCGGGCAAGTCAGACCGTTAGCATCTATTTAAATTGGTATCAGCAAAAA25630.1611668910510559No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24810.15601055665145128No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23540.14802452654474663No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT23500.14777299803744884No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23400.14714417676920435No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCCTCACTTGCCGGGCAAGTCAGACCGTTAGCAT22530.14167343173547753No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22510.14154766748182865No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT22470.14129613897453083No Hit
GAGTGAATTCTGTCCCAGATCCACGGCCACTGAACGTTGATGGGACCCCA22070.138780853901553No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21770.1368943900968196No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC21150.13299569823370394No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG20940.13167517357039057No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20590.12947429913153494No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGGTCACGTTCAGGGAGAACTGGTTCTTGGACTTGTCTACTGA19700.12387778984415923No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT19350.1216769154053036No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC19150.12041927286881467No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19010.11953892309327244No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18460.11608040611792787No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18430.11589175973745454No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17710.11136424660609441No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17330.10897472578676545No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17300.1087860794062921No Hit
GTGGGGTCCCATCAACGTTCAGTGGCCGTGGATCTGGGACAGAATTCACT17210.10822014026487209No Hit
ATAGTAGGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACGGCCA17190.10809437601122321No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16870.10608214795284092No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16720.10513891605047423No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16570.10419568414810752No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC16240.1021205739629008No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16230.10205769183607635No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCCTCACTTGCCGGGCAAGTCAGA16190.10180616332877857No Hit
GAGTCACCCTCACTTGCCGGGCAAGTCAGACCGTTAGCATCTATTTAAATTGGTATCAGCAAAAACCAGGGAAGG16160.10161751694830524No Hit
ACCTACTATTGTCTGCAGACTTATACTCCACCTAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA16150.10155463482148079No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16110.101303106314183No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16040.10086293142641187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6300.044.010481
GTATCAA43950.036.9287951
GACAGGT3350.033.3082581
TATCAAC50800.031.8120982
TCAACGC51900.030.924914
AACGCAG52050.030.8977936
CAACGCA51950.030.8922315
CGCAGAG54550.029.6056338
ACGCAGA54750.029.4357767
GCAGAGT55950.028.9243099
AGAGTAC55800.028.87915211
TACGTAC953.846253E-728.45345112
CCGTATC500.006762319228.37093755
GCCGTAT500.00684666328.29901154
ATACGTA1006.030514E-727.02652511
GAGTTTA5850.024.8539491
AGTTTAT5900.024.6402192
GTGCTAG1651.4915713E-1024.591221
AATACGT1101.3843201E-624.57034110
GAGTACG52450.023.64226712
Produced by FastQC (version 0.11.5)