FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1044621
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30480.2917804639194502No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG30220.28929152295425803No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24910.23845969016514124No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22150.21203862453463984No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21760.20830521308685157No Hit
GTAATACACAGCCATGTCCTCAGCTCTCAGGCTGCCCATTTGAAGATACA21070.20169994667922625No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20460.1958605082608908No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20430.19557332276490708No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19970.19116981182649018No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19710.188680870861298No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19370.18542610190681597No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18780.179778120485803No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17300.16561030268393992No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG17270.1653231171879562No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC17090.16360000421205392No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC16680.15967513576694323No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG16580.15871785078033085No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16380.15680328080710612No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15660.14991082890349705No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15260.1460816889570476No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA15240.1458902319597251No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15140.14493294697311274No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC14690.14062516453335708No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14670.1404337075360346No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14270.1366045675895851No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14240.13631738209360142No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14220.13612592509627894No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT14130.1352643686083278No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13640.13057367217392718No Hit
GTAGTAGTGATCATCCGGATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCC13240.12674453222747772No Hit
GGATAGTAGTAGTGATCATCCGGATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG13090.12530860474755917No Hit
CACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGGGTCA12920.12368122027031814No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATCCGGATGATCACTACTACTATCC12710.12167092179843217No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT12350.11822469584662762No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12330.11803323884930517No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12070.115544297884113No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC12060.11544856938545175No Hit
ATGCAACATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGG11860.11353399941222701No Hit
CGGTTAGTTTGCATGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGG11790.11286389992159836No Hit
ACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGA11490.10999204496176125No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11460.10970485946577754No Hit
GGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTGTCGGACATGACCAGGGA11440.10951340246845506No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT11400.10913048847381011No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG11030.10558853402334435No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATCCGGATGA10980.10510989153003816No Hit
CCCCGAGCCTGTGGGCAGGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTG10880.10415260654342579No Hit
GGTCAGTAGCAGGTGCCAGCTGTGTCGGACATGACCAGGGACACGTTGTA10840.10376969254878084No Hit
GTGTCGGACATGACCAGGGACACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACC10760.10300386455949095No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10720.102620950564846No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10670.10214230807153982No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC10590.10137648008224993No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10560.10108929458626621No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGGG8650.043.2016970
TGGTATC5000.043.11532
GGTATCA14600.040.5698931
GTATCAA36100.039.64421
GACCGGA1201.314445E-534.6013570
TTAACGA501.923598E-434.3072330
TATCAAC41600.034.300752
ATCAACG42550.033.3597073
TCAACGC43000.032.846794
CAACGCA43900.032.248215
AACGCAG44800.031.9056846
CGCAGAG48450.029.854958
ACGCAGA48400.029.6738367
GTATAGG704.0561008E-529.3470081
CAGAGTA50050.028.76389910
AGAGTAC49700.028.75867511
GCAGAGT50200.028.6779529
AAACACG500.00704620828.132157
GTACGAG500.008067002527.3629993
GACAGGT2150.027.0720441
Produced by FastQC (version 0.11.5)