FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193134
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT6500.3365538952230058No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC5410.2801163958702248No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4860.2516387585821243No Hit
GTAATAGTCAGCCTCGTCCTCAATCTGGAGCCCAGAGATGGTCAGGGAGG4820.2495676576884443No Hit
GACTATTACTGCGCCTCATATGAATACAAGGGCGCAGCCTTCGGACCTGGGACCAAAGTCACCGTCCTACGTCAG4340.22471444696428386No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACGCTAGTGTGTCTGATCAGT4320.22367889651744385No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT4180.21643004338956373No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTAGGCTGA3930.2034856628040635No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC3740.19364793355908333No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC3420.17707912640964305No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT3290.17034804850518293No Hit
GCCTAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC3040.15740366791968272No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC2870.14860148912154256No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2850.14756593867470252No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCAGTGACGTCGGAACTGACAACCATGTCTCCTGGTTTCAGCAACAGC2760.14290596166392247No Hit
CTATTACTGCGCCTCATATGAATACAAGGGCGCAGCCTTCGGACCTGGGACCAAAGTCACCGTCCTACGTCAGC2650.13721043420630236No Hit
CACTAGCGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTAGG2550.13203268197210227No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT2470.1278904801847422No Hit
GTCGATCACCATCTCCTGCAGTGACGTCGGAACTGACAACCATGTCTCCTGGTTTCAGCAACAGCCAGGCAAAGC2420.12530160406764215No Hit
CAGTAATAGTCAGCCTCGTCCTCAATCTGGAGCCCAGAGATGGTCAGGGA2410.12478382884422215No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG2380.12323050317396213No Hit
GCCTTCGGACCTGGGACCAAAGTCACCGTCCTACGTCAGCCTAAGGCCAACCCCACTGTCACTCTGTTCCCGCC2380.12323050317396213No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT2340.12115940228028209No Hit
GCCTTAGGCTGACGTAGGACGGTGACTTTGGTCCCAGGTCCGAAGGCTGC2140.11080389781188191No Hit
GTAATATGTGGCCGTGTCCGCAGCGGTCACAGAGGTCATCTTCAGGGAGAACTGGTTCTTGGACGTGTCTGCTGA2120.10976834736504189No Hit
CTCTTAATTTATGAAGTCAGTCATCGGCCCTCAGGCGTCTCTAATCGGTT2070.10717947124794183No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT2050.10614392080110183No Hit
GTGTAGGGGCTTTGCCTGGCTGTTGCTGAAACCAGGAGACATGGTTGTCA2050.10614392080110183No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC2010.10407281990742179No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC2000.10355504468400178No Hit
GACTCAGCCTGCCTCCGTGGCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCAGTGACGTCGGAACTGACAA1950.10096616856690174No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)