FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193134
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25031.2959913842202824No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC4310.22316112129402385No Hit
GACTATTACTGCGCCTCATATGAATACAAGGGCGCAGCCTTCGGACCTGGGACCAAAGTCACCGTCCTACGTCAG3820.1977901353464434No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT3690.1910590574419833No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTAGGCTGA3440.17811467685648308No Hit
GTAATAGTCAGCCTCGTCCTCAATCTGGAGCCCAGAGATGGTCAGGGAGG3420.17707912640964305No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC3190.16517029627098284No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACGCTAGTGTGTCTGATCAGT2980.15429701657916267No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC2960.15326146613232264No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCAGTGACGTCGGAACTGACAACCATGTCTCCTGGTTTCAGCAACAGC2850.14756593867470252No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC2710.1403170855468224No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG2690.1392815350999824No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT2640.13669265898288235No Hit
GTCGATCACCATCTCCTGCAGTGACGTCGGAACTGACAACCATGTCTCCTGGTTTCAGCAACAGCCAGGCAAAGC2580.1335860076423623No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGGCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG2540.13151490674868227No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT2460.1273727049613222No Hit
CTATTACTGCGCCTCATATGAATACAAGGGCGCAGCCTTCGGACCTGGGACCAAAGTCACCGTCCTACGTCAGC2360.1221949527271221No Hit
CACTAGCGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTAGG2340.12115940228028209No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGGCTGGGTCTCCTGGACAGTCGATCAC2310.11960607661002207No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG2250.116499425269502No Hit
GGCTCAGGTAGCTGCTGGCCGCGTATTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA2210.11442832437582195No Hit
CTCTTAATTTATGAAGTCAGTCATCGGCCCTCAGGCGTCTCTAATCGGTT2100.10873279691820187No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA2030.10510837035426181No Hit
GCCTAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC1940.10044839334348173No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)