FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296183
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29340.9906037821211886No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8990.30352856173379295No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA8330.28124504107258014No Hit
GAGTTACACTTCCCCGCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT7420.25052079288818063No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7290.24613161457612354No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC7010.23667799975015447No Hit
GTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGTAGACTGCTGATGGTGAGAGTGAAATCTGTC6770.22857490132789526No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6060.20460323516204507No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA5720.1931238457305112No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTCCAGGGAGA5710.19278621662958373No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG5460.18434548910639706No Hit
GTCTACAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACACTTCCCCGCACACTTTTGGCCAGGGGA5380.18164445629897732No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT4990.1684769213628061No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG4930.16645114675724132No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4850.16375011394982156No Hit
GGCCAAAAGTGTGCGGGGAAGTGTAACTCTGTTGACAGTAGTAAGTTGCA4840.1634124848488941No Hit
CATCTATGCTGCATCCAGTTTGCAGAGTGGGGTCCCATCAAGGTTCAGTGGCAGCGGATCTGGGACAGATTTCA4820.16273722664703916No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG4500.1519330954173602No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT4240.14315473879324608No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4230.14281710969231862No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG4100.13842793138026155No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT4000.13505164037098685No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT3930.13268823666449459No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA3780.12762380015058256No Hit
ACTTACTACTGTCAACAGAGTTACACTTCCCCGCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA3720.12559802554501778No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC3690.12458513824223538No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG3660.12357225093945297No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT3600.12154647633388818No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG3590.12120884723296071No Hit
GATTTCACTCTCACCATCAGCAGTCTACAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACACTTCC3580.12087121813203322No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT3570.12053358903110575No Hit
GAGTGAAATCTGTCCCAGATCCGCTGCCACTGAACCTTGATGGGACCCCA3570.12053358903110575No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC3450.11648203981997615No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG3420.11546915251719377No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG3420.11546915251719377No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA3410.1151315234162663No Hit
GCATAGATGAGGAGCTTAGGGGCTTTCCCTAGTTTCTGCTGATACCAATT3400.11479389431533883No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCGTTAGCATCTATTTAAATTGGTATCAGCAGAAA3370.11378100701255642No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG3310.11175523240699163No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGTAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCGCTGCCA3290.1110799742051367No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCGTTAGCAT3150.10635316679215215No Hit
GTGTATTACTGTGCGAGACGGGGGGCTGGACAGACCAAGTTTACTTACTTCTTTGACTACTGGGGCCAGGGAACC3130.10567790859029721No Hit
GTTGTAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCGCTGCCACTGAACCTTGATGGGACCCCACT3120.10534027948936975No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC3060.10331450488380493No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG3060.10331450488380493No Hit
GCTTAGGGGCTTTCCCTAGTTTCTGCTGATACCAATTTAAATAGATGCTA3050.10297687578287747No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)