FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483814
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTTCAGCTCTCGGGCTGTTCATTTGCAGATACA40740.27456271473378735No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39140.26377969206383006No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38440.2590621196457238No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28430.19160083406680353No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27380.18452447543964406No Hit
GTGTATTACTGTGCGAAAGATCTACGGATACAGCTATCGTATTATTTTGA27040.18223308312227812No Hit
GGCTAACAGTTTCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT26960.18169393198878028No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26590.17920035799635264No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26380.17778508627092077No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25880.17441539168655912No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24380.16430630793347414No Hit
GAGTATTAGGAGCTGGTTAGCCTGGTATCAGCAGATACCTGGTAAAGCCCCTACGCTCCTGATGTATGGTGCATC23330.15722994930631468No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23250.1566907981728168No Hit
CTGTTAGCCTGTTGACAATAGTAAGTCGCAAAATCTTCAGGCTGCAGGCTACTGATAGTGAGAGTGAAATCTGTC23240.15662340428112959No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21850.14725565333660418No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21530.1450990488026127No Hit
GTATTAGGAGCTGGTTAGCCTGGTATCAGCAGATACCTGGTAAAGCCCCT21260.14327941372705744No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGAGTATTAGGAGCTGGTTAGCCTGGTATCAGCAGATA21150.14253808091849787No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC21140.14247068702681065No Hit
CACTATCAGTAGCCTGCAGCCTGAAGATTTTGCGACTTACTATTGTCAACAGGCTAACAGTTTCCCTCGGACGTT21070.141998929785No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21070.141998929785No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20220.1362704489915852No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19660.13249639105710015No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19660.13249639105710015No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19080.12858754533924063No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18710.126093971346813No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18520.12481348740475558No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18370.1238025790294471No Hit
CCTGATGTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTT18360.12373518513775984No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCGAGGGAAA18330.12353300346269816No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC17620.1187480371529046No Hit
GATGTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA17500.1179393104526578No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATCCCATAACTAC17310.11665882651060039No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17010.1146370097599834No Hit
CAGTAATACACAGCCGTGTCTTCAGCTCTCGGGCTGTTCATTTGCAGATA16990.11450222197660893No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16490.11113252739224729No Hit
GTATTACTGTGCGAAAGATCTACGGATACAGCTATCGTATTATTTTGACTCCTGGGGCCAGGGAACCCTGGTCAC16160.10890852896656858No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT16020.10796501448294732No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT15960.10756065113282393No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15920.10729107556607499No Hit
CTCCTGATGTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT15660.10553883438220693No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15440.1040561687650878No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15310.10318004817315377No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGTTATGGGATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG15060.10149520088097296No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14880.10028211083060276No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14840.10001253526385383No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACA200.00727432451.9024642
CCGTAAC200.007308958651.84014541
TCCTATA306.761126E-445.362827
GTGGTAT4200.044.4761541
ACCGATC4550.041.87335627
CGTATGC1850.041.09849541
GACCGAT4650.040.2221826
CGATCAG5000.039.5176829
TCAGTAT4800.039.1223732
CTTATAC4950.039.109611
CCGATCA5100.038.7166428
TATCGCG901.3096724E-1038.09174736
TATCTCG2350.037.92965736
GTATCAA41400.037.7373471
GTATCGC1001.0913936E-1137.67109735
TCTGGAG15750.037.24989370
ATCGCGT655.667098E-736.9712737
ACGAGAC5150.036.93005822
AGTATCG1950.036.8382234
TCTCCGA5550.036.70380811
Produced by FastQC (version 0.11.5)