FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483814
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56960.3838756070504794No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39460.26593629659782153No Hit
GTAATACACAGCCGTGTCTTCAGCTCTCGGGCTGTTCATTTGCAGATACA36280.24450503904128149No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27630.1862093227318249No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27010.18203090144721643No Hit
GTGTATTACTGTGCGAAAGATCTACGGATACAGCTATCGTATTATTTTGA26550.17893078242960372No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25940.1748197550366825No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24600.16578897355059327No Hit
GGCTAACAGTTTCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT24080.16228449118285715No Hit
CTGTTAGCCTGTTGACAATAGTAAGTCGCAAAATCTTCAGGCTGCAGGCTACTGATAGTGAGAGTGAAATCTGTC24050.16208230950779545No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22150.14927747008722117No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20340.137079175691832No Hit
GAGTATTAGGAGCTGGTTAGCCTGGTATCAGCAGATACCTGGTAAAGCCCCTACGCTCCTGATGTATGGTGCATC19600.13209202770697676No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19460.1311485132233555No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19170.12919409036442575No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18970.12784621253068107No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18890.1273070613971832No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18810.12676791026368533No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC18640.12562221410500238No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18010.1213763989287067No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17920.12076985390352162No Hit
CACTATCAGTAGCCTGCAGCCTGAAGATTTTGCGACTTACTATTGTCAACAGGCTAACAGTTTCCCTCGGACGTT17720.11942197606977693No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC17580.11847846158615567No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGAGTATTAGGAGCTGGTTAGCCTGGTATCAGCAGATA16930.11409785862648553No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCGAGGGAAA16800.11322173803455152No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATCCCATAACTAC16500.11119992128393451No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16330.11005422512525155No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG16280.10971725566681538No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16160.10890852896656858No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16100.10850416561644519No Hit
GTATTACTGTGCGAAAGATCTACGGATACAGCTATCGTATTATTTTGACTCCTGGGGCCAGGGAACCCTGGTCAC15910.10722368167438777No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15890.1070888938910133No Hit
GTATTAGGAGCTGGTTAGCCTGGTATCAGCAGATACCTGGTAAAGCCCCT15840.10675192443257712No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15790.10641495497414097No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15780.10634756108245373No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15560.1048648954653346No Hit
CCTGATGTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTT15540.10473010768196013No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15480.10432574433183674No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15210.10250610925628145No Hit
CTCCTGATGTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT14990.10102344363916232No Hit
GATGTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA14970.10088865585578786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5200.045.6703451
TCTGGAG15950.043.11948870
GTATCAA42550.036.7570761
GACAGGT2500.032.569481
TATCAAC49400.031.6484452
ATCAACG50950.030.8697053
TCAACGC51850.030.3256994
AACGCAG51950.030.1980236
CAACGCA52050.030.1410185
ACGCAGA54100.029.0605457
CGCAGAG54900.028.5753578
AGAGTAC55300.028.30548711
GTACGGT606.017169E-428.24352514
CGTGTCT14700.028.12634813
GCAGAGT56050.028.0495199
GTTCATT15500.027.36096834
GTTCGTG657.5204944E-427.18625655
CCGTGTC15300.027.01971212
GCTCTCG15250.026.91876223
ACAGGTA2900.026.9072571
Produced by FastQC (version 0.11.5)