FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1756538
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATAGGGCCGTGTCCTCGCCTCTCAGGCTGTTCATTTGGAGATACA49690.2828859950652932No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG44890.25555951536488253No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43900.2499234289266728No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35790.20375306426618725No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA34790.19806004766193502No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA33900.19299326288415053No Hit
CAGTAATATAGGGCCGTGTCCTCGCCTCTCAGGCTGTTCATTTGGAGATA32280.18377057598526192No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT31550.1796146738641578No Hit
ATATTACTGTGCGAGAAGGGGGTATAACTGGAACTCGCCTGACTGCTGGGGCCAGGGAACCCTGGTCACCGTCTC31430.17893151187164752No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG30940.17614193373556392No Hit
CTATATTACTGTGCGAGAAGGGGGTATAACTGGAACTCGCCTGACTGCTGGGGCCAGGGAACCCTGGTCACCGT29680.16896873281420613No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29070.16549599268561227No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAG27480.15644409628485123No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27360.15576093429234097No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTCTG26650.15171889250332188No Hit
GTCTGGGACAGACTTCATTCTCACCATCAGCAGCCTAGAGCCTGAAGACT26220.14927089536349342No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC26170.1489862445332808No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26080.1484738730388981No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC24620.14016206879668985No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT24440.13913732580792446No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAGCCTCTTAGCCTGGTA23920.1361769571737133No Hit
GTTTATTACTGTCACCAGCGTCGCAGCTGGCCCATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA23760.13526607451703293No Hit
CTCCTGTTCAGCCTCTGGATTCACCTTTAGCAACTATGCCATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG23710.1349814236868203No Hit
GTAATAAACTGCAAAGTCTTCAGGCTCTAGGCTGCTGATGGTGAGAATGAAGTCTGTCCCAGACCCACTGCCA23430.1333873790376297No Hit
GCCTAGAGCCTGAAGACTTTGCAGTTTATTACTGTCACCAGCGTCGCAGCTGGCCCATCACCTTCGGCCAAGGGA22820.12991463890903585No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22670.12906068641839802No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAGCCTCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT22670.12906068641839802No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22430.12769436243337748No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22290.12689734010878218No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC21740.12376618097644343No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21660.12331073964810327No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT21460.12217213632725282No Hit
GTCTTCAGGCTCTAGGCTGCTGATGGTGAGAATGAAGTCTGTCCCAGACC21400.12183055533099768No Hit
GAGTGTTAGTAGCCTCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC20830.1185855358665739No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20810.11847167553448885No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20800.11841474536844634No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20680.11773158337593607No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20610.11733307221363841No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG20580.11716228171551085No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20200.11499893540589501No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT19310.10993215062811053No Hit
GACTTCATTCTCACCATCAGCAGCCTAGAGCCTGAAGACTTTGCAGTTTATTACTGTCACCAGCGTCGCAGCTGG18950.10788266465057972No Hit
GCCCTATATTACTGTGCGAGAAGGGGGTATAACTGGAACTCGCCTGACTG18400.104751505518241No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT18220.1037267625294756No Hit
GAATGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA18200.10361290219739056No Hit
GTGTTAGTAGCCTCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCC18160.10338518153322046No Hit
CTCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCCAGAGGGCCA18150.10332825136717794No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC18130.10321439103509289No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGATA100.007835157140.2374170
TGGTATC3750.044.42032
GTGGTAT4000.043.3462561
TCGTATG3750.040.44153240
CGTATGC3700.040.13064241
GTATCAA31400.040.0602341
GGTATCA11500.039.9096031
TAACGGC801.7407729E-938.58291236
GTCGTAT655.5540295E-737.06717339
ATCTCGT3250.036.96592737
AATCTCG3250.036.93406736
GTATGCC4150.036.6546742
ATGCCGT4100.035.51603344
TGCCGTC4000.034.72688745
GCCGTCT3850.034.3218146
TATCAAC36750.034.3188932
TATACAC5000.033.9951323
ATCAACG37800.033.4555243
CTTATAC5500.032.760811
ACGAGAC5100.032.65826822
Produced by FastQC (version 0.11.5)